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US 12,613,204 B2Granted
Method for analyzing endosomal escape efficiency of drug delivery particles
AssigneeSeoul National University R&Db Foundation
InventorsHyung Jun Im, Jeong Bin Park, So Min Lee
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Abstract
A method for quantitatively analyzing endosomal escape efficiency of drug delivery particles includes measuring magnetic resonance signals (MR signals) reflected upon emission of electromagnetic waves (for example, radio waves) into cells having iron oxide-carrying particles ingested thereinto, with the aid of a magnetic resonance imaging device, and obtaining a change rate of magnetic relaxation with time from the measurements.
Claims6
A method for analyzing endosomal escape efficiency of drug delivery particles, the method comprising:
- measuring, in a cell into which particles having iron oxides loaded therein are ingested, a magnetic resonance signal which changes as the iron oxides are released from the particles to the cell;
- obtaining a graph representing changes in a magnetic relaxation rate due to a lapse of time from the magnetic resonance signal;
- obtaining a change rate of the magnetic relaxation rate in an arbitrary time interval of the graph;
- and
- obtaining an endosomal escape rate of the particles by substituting the change rate of the magnetic relaxation rate into Equation 1 below:
- Endosomal escape rate (%)=(Change rate of magnetic relaxation rate in cell having particles ingested thereinto÷(Magnetic relaxation rate of total iron oxides loaded in particles before endosomal escape−Magnetic relaxation rate of total free iron oxides released from particles due to the endosomal escape))×100 [Equation 1].
3 more elements
Dependencies are inferred from the claim text.