Particle isolation device, particle isolation method, and program

A particle isolation method comprising:

• a vibration step of applying vibration to a fluid containing sheath liquid by supplying a driving voltage based on each of a plurality of frequencies;
• an imaging step of acquiring, at a position where the fluid is formed into droplets through the vibration, an image of the fluid and each of the droplets;
• a liquid temperature control step of controlling a liquid temperature of the sheath liquid;
• and
• a frequency control step of acquiring data regarding a state of the droplet at each of the frequencies per liquid temperature of the sheath liquid from the image captured by the imaging step, and controls the frequency of the driving voltage on a basis of a variation in the data accompanying a change in the liquid temperature of the sheath liquid.

A program for causing a particle isolation device to execute functions of:

• applying vibration to a fluid containing sheath liquid by supplying a driving voltage based on each of a plurality of frequencies;
• acquiring, at a position where the fluid is formed into droplets through the vibration, an image of the fluid and each of the droplets;
• controlling a liquid temperature of the sheath liquid;
• and
• acquiring data regarding a state of the droplet at each of the frequencies per liquid temperature of the sheath liquid from the image captured, and controlling the frequency of the driving voltage on a basis of a variation in the data accompanying a change in the liquid temperature of the sheath liquid.