Label-free electrical monitoring of cell aggregates

Abstract

A method of monitoring one or more cell aggregates, includes providing a flow path in which the one or more cell aggregates are in a medium and the flow path being configured to pass through a collective sensing zone of a set of electrodes, obtaining impedance-related signals corresponding to each of the medium and one of the one or more cell aggregates in the medium, and determining one or more electrical signatures for a cell aggregate, in which the one or more electrical signatures are based on impedance-related signals obtained from the set of electrodes. The method is one of dynamic testing at single-particle resolution. The electrical signatures may be an opacity and/or electrical size of the one or more cell aggregates, or electrical impedance spectroscopy-based electrical signatures. It is also to provide a microfluidic chip having a channel and electrodes for obtaining impedance-related signals.

Dependencies are inferred from the claim text.