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AssigneePivot Bio, Inc.
InventorsMin-Hyung Ryu, Bilge Ozaydin Eskiyenenturk, Alvin Tamsir
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The present disclosure provides high-throughput methods for rapidly mutagenizing, screening, and targeting candidate microbes that are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The methods utilize a microbial biosensor capable of detecting the presence/absence of ammonium and/or glutamine in a composition and signaling with a fluorescent reporter. The present disclosure further utilizes rapid visual detection assays capable of processing thousands of candidate microbes. The disclosed methods and biosensor can be used to identify mutant bacteria with improved nitrogen fixing capabilities. Mutant bacteria with improved nitrogen fixing capabilities are also disclosed, as well as methods of utilizing these novel bacteria to provide fixed nitrogen to a plant.
A nitrogen fixing bacterium comprising a mutant GlnA protein comprising an amino acid sequence with an N339D amino acid substitution at amino acid position 339 of a Klebsiella GlnA protein with SEQ ID NO:
A nitrogen fixing bacterium comprising a mutant glnA gene comprising a nucleotide substitution at nucleotide position 1015 of a Klebsiella glnA gene with SEQ ID NO:
A nitrogen fixing bacterium comprising a mutant glnA gene comprising a nucleotide substitution at nucleotide position 1015 of a Klebsiella glnA gene with SEQ ID NO:
A microbial composition, comprising:
Dependencies are inferred from the claim text.