SNP markers and selection of low fiber in brassica

A method for producing a Brassica napus plant comprising a low fiber content trait, the method comprising:

• a. isolating or providing a nucleic acid sample from each of one or more Brassica napus plants or germplasm thereof;
• b. screening each sample for and detecting the presence of one or more low fiber content nucleic acid marker alleles located within Brassica napus chromosome N13 interval between marker DBSNP143552 in SEQ ID NO:1 and marker DBSNP243314 in SEQ ID NO:89, wherein the one or more detected marker alleles comprise one or more of an A at position 220 of SEQ ID NO:1, A at position 101 of SEQ ID NO:2, C at position 61 of SEQ ID NO:3, T at position 151 of SEQ ID NO:4, C at position 151 of SEQ ID NO:5, A at position of 101 of SEQ ID NO:6, C at position 86 of SEQ ID NO:7, C at position 151 of SEQ ID NO:8, G at position 151 of SEQ ID NO:9, G at position 151 of SEQ ID NO:10, G at position 151 of SEQ ID NO:11, T at position 150 of SEQ ID NO:12, C at position 151 of SEQ ID NO:13, C at position 151 of SEQ ID NO:14, G at position 151 of SEQ ID NO:15, T at position 151 of SEQ ID NO:16, C at position 219 of SEQ ID NO:17, T at position 250 of SEQ ID NO:18, A at position 61 of SEQ ID NO:19, G at position 151 of SEQ ID NO:20, T at position 197 of SEQ ID NO:21, G at position 61 of SEQ ID NO:22, A at position 151 of SEQ ID NO:23, T at position 151 of SEQ ID NO:24, T at position 151 of SEQ ID NO:25, C at position 151 of SEQ ID NO:26, G at position 151 of SEQ ID NO:27, C at position 151 of SEQ ID NO:28, A at position 151 of SEQ ID NO:29, G at position 61 of SEQ ID NO:30, A at position 151 of SEQ ID NO:31, C at position 380 of SEQ ID NO:32, T at position 61 of SEQ ID NO:33, C at position 151 of SEQ ID NO:34, A at position 53 of SEQ ID NO:35, C at position 61 of SEQ ID NO:36, T at position 61 of SEQ ID NO:37, T at position 61 of SEQ ID NO:38, A at position 61 of SEQ ID NO:39, T at position 61 of SEQ ID NO:40, C at position 151 of SEQ ID NO:41, A at position 151 of SEQ ID NO:42, T at position 151 of SEQ ID NO:43, T at position 151 of SEQ ID NO:44, T at position 151 of SEQ ID NO:45, G at position 151 of SEQ ID NO:46, G at position 61 of SEQ ID NO:47, A at position 151 of SEQ ID NO:48, G at position 151 of SEQ ID NO:49, G at position 151 of SEQ ID NO:50, T at position 151 of SEQ ID NO:51, G at position 61 of SEQ ID NO:52, T at position 61 of SEQ ID NO:53, T at position 424 of SEQ ID NO:54, C at position 151 of SEQ ID NO:55, C at position 61 of SEQ ID NO:56, T at position 61 of SEQ ID NO:57, T at position 61 of SEQ ID NO:58, A at position 151 of SEQ ID NO:59, C at position 101 of SEQ ID NO:60, T at position 301 of SEQ ID NO:61, T at position 370 of SEQ ID NO:62, T at position 151 of SEQ ID NO:63, G at position 151 of SEQ ID NO:64, A at position 151 of SEQ ID NO:65, G at position 151 of SEQ ID NO:66, G at position 101 of SEQ ID NO:67, C at position 151 of SEQ ID NO:68, A at position 151 of SEQ ID NO:69, G at position 151 of SEQ ID NO:70, T at position 151 of SEQ ID NO:71, G at position 151 of SEQ ID NO:72, A at position 151 of SEQ ID NO:73, T at position 151 of SEQ ID NO:74, G at position 151 of SEQ ID NO:75, G at position 151 of SEQ ID NO:76, C at position 61 of SEQ ID NO:77, G at position 61 of SEQ ID NO:78, A at position 151 of SEQ ID NO:79, G at position 151 of SEQ ID NO:80, C at position 61 of SEQ ID NO:81, A at position 61 of SEQ ID NO:82, T at position 61 of SEQ ID NO:83, G at position 151 of SEQ ID NO:84, A at position 61 of SEQ ID NO:85, G at position 61 of SEQ ID NO:86, T at position 501 OF SEQ ID NO:87, T at position 101 of SEQ ID NO:88, A at position 101 of SEQ ID NO:89, T at position 101 of SEQ ID NO:90, T at position 201 of SEQ ID NO:95, or C at position 90 of SEQ ID NO:100;
• c. selecting a first Brassica napus plant or germplasm thereof identified as having the one or more low fiber content marker alleles screened for in step b;
• and
• d. crossing the first plant selected in step c. with a second plant to produce progeny plants, wherein at least one of the progeny plants comprises the one or more low fiber content marker alleles.