US 12,612,653 B2 · Polypeptides for producing albicanol and/or drimenol compounds

Abstract

Described herein are plant-derived haloacid dehalogenase-like (HAD-like) polypeptides having cyclic terpene synthase (TPS) activity, in particular suitable for use in biochemical methods of producing drimane-type sesquiterpenes, encompassing drimenol and/or albicanol and related compounds, like phosphorylated drimane-type sesquiterpene alcohols, in particular phosphorylated drimenol and/or albicanol compounds and derivatives. Also described herein are the coding nucleotide sequences of the TPS activity, corresponding expression constructs, recombinant hosts, and methods of preparing such polypeptides and mutants and variants thereof. Also described herein is a method of using such polypeptides, the method including using the polypeptides in the production of odorants, flavours and fragrance ingredients.

Claims4

Dependent claims

A method for producing a drimane sesquiterpene, comprising:

a. contacting farnesyl diphosphate (FPP) with an isolated polypeptide of the haloacid dehalogenase-like (HAD-like) hydrolase superfamily comprising cyclic terpene synthase activity having an, amino acid sequence with at least 70% sequence identity to SEQ ID NO:
3, 6, 9, 12, 19, or 26 and wherein the amino acid sequence does not include a class I motif as set forth in SEQ ID NO:
45 (DDxx(D/E), thereby obtaining at least one diphosphate of drimane sesquiterpene;
b. chemically or enzymatically cleaving the diphosphate moiety of said at least one diphosphate of drimane sesquiterpene obtained in step a.;
and
c. optionally isolating the drimane sesquiterpene.

3 more elements

Dependencies are inferred from the claim text.

Polypeptides for producing albicanol and/or drimenol compounds

Life of this patent

Polypeptides for producing albicanol and/or drimenol compounds

Life of this patent

Abstract

Claims4