Methods and compositions for increasing harvestable yield via editing GA20 oxidase genes to generate short stature plants
AssigneeMonsanto Technology LLC
InventorsSivalinganna Manjunath, Linda A. Rymarquis, Thomas L. Slewinski
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Abstract
The present disclosure provides compositions and methods for the editing or mutating of specific subtypes of GA20 oxidase genes and specific zygosity combinations of those edits or mutations. Modified plants, and plant parts and cells thereof, having mutations reducing the expression or activity of GA20 oxidase genes are further provided with improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types. Methods are further provided for making modified plants, and plant parts and cells thereof, having one or more mutations in specific subtypes of GA20 oxidase genes.
Claims38
A modified corn plant, or plant part thereof, comprising a mutant allele of a GA20 oxidase_3 gene at the GA20 oxidase_3 locus, wherein a wild-type allele of the GA20 oxidase_3 gene encodes a GA20 oxidase_3 protein comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:
- 9, and a mutant allele of a GA20 oxidase_5 gene at the GA20 oxidase_5 locus, wherein a wild-type allele of the GA20 oxidase_5 gene encodes a GA20 oxidase_5 protein comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:
- 15, and wherein
- (i) said modified corn plant is homozygous for the mutant allele at said GA20 oxidase_3 locus and is heterozygous for the mutant allele at said GA20 oxidase_5 locus, or
- (ii) said modified corn plant is heterozygous for the mutant allele at said GA20 oxidase_3 locus and is homozygous for the mutant allele at said GA20 oxidase_5 locus, and
- wherein said modified corn plant has a shorter plant height relative to a wild-type control plant.
A method of making a modified progeny corn plant, or plant part thereof, comprising:
- (a) crossing a first corn plant comprising a mutant allele of a GA20 oxidase_3 gene at the GA20 oxidase_3 locus, wherein a wild-type allele of the GA20 oxidase_3 gene encodes a GA20 oxidase_3 protein comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:
- 9, with a second corn plant comprising a mutant allele of a GA20 oxidase_5 gene at the GA20 oxidase_5 locus, wherein a wild-type allele of the GA20 oxidase_5 gene encodes a GA20 oxidase_5 protein comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:
- 15;
- and
- (b) selecting a progeny corn plant, or plant part thereof, from the cross in step (a) that is
- (i) homozygous for one or more mutant alleles at the GA20 oxidase_3 locus and heterozygous for a mutant allele at the GA20 oxidase_5 locus, or
- (ii) heterozygous for a mutant allele at the GA20 oxidase_3 locus and homozygous for one or more mutant alleles at the GA20 oxidase_5 locus,
- wherein the progeny corn plant has a shorter plant height relative to a wild-type control plant.
Dependencies are inferred from the claim text.