US 12,612,640 B2Granted

Gene related to biosynthesis of ergothioneine, and use thereof

AssigneeShanghai Academy of Agricultural Sciences

InventorsJianjie Gao, Yongdong Deng, Quanhong Yao, Rihe Peng, Yongsheng Tian, Bo Wang, Zhenjun Li, Jing Xu, Hongjuan Han, Lijuan Wang, Xiaoyan Fu, Yu Wang, Wenhui Zhang, Zhihao Zuo, Cen Qian

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Abstract

A gene related to biosynthesis of ergothioneine is provided. Based on five synthesis genes of ergothioneine in Mycobacteroides abscessus, only an encoding sequence of each of the five genes is retained, and a gene structure of an encoding region in each gene is optimized to obtain genes related to the biosynthesis of the ergothioneine that are stably expressed in rice. These genes have sequences set forth in SEQ ID NO: 1 to SEQ ID NO: 5, and encoded proteins thereof have amino acid sequences set forth in SEQ ID NO: 6 to SEQ ID NO: 10. The gene is transferred into rice to construct a transgenic rice molecular farm to produce the ergothioneine. As a molecular farm, rice seeds do not contain alkaloids or allergens that are harmful to the human body. Moreover, biosynthesized ergothioneine shows the advantages of easy extraction and processing and of low production cost.

Claims15

Dependent claims

A gene related to biosynthesis of ergothioneine, wherein the gene is one or more selected from the group consisting of an EgtA gene, an EgtB gene, an EgtC gene, an EgtD gene, and an EgtE gene;

wherein:
the EgtA gene has the nucleotide sequence set forth in SEQ ID NO:
1 or a nucleotide sequence having at least 95% sequence identity to SEQ ID NO:
1;
the EgtB gene has the nucleotide sequence set forth in SEQ ID NO:
2 or a nucleotide sequence having at least 95% sequence identity to SEQ ID NO:
2;
the EgtC gene has the nucleotide sequence set forth in SEQ ID NO:
3 or a nucleotide sequence having at least 95% sequence identity to SEQ ID NO:
3;
the EgtD gene has the nucleotide sequence set forth in SEQ ID NO:
4 or a nucleotide sequence having at least 95% sequence identity to SEQ ID NO:
4;
and the EgtE gene has the nucleotide sequence set forth in SEQ ID NO:
5 or a nucleotide sequence having at least 95% sequence identity to SEQ ID NO:
5.

13 more elements

Dependencies are inferred from the claim text.

Facts

Application: 18/772,908Filed 15 Jul 2024
Priority: CN 202311036218.817 Aug 2023
Published: US 2025/0059553 A120 Feb 2025
Granted: 28 Apr 2026
Term ends: Not estimatedThe source records don’t support an estimate.

Classification14 codes

C12N 15/8251
Involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine: amino acid content, e.g. synthetic storage…
C12N 9/0083
Acting on paired donors with incorporation of molecular oxygen: miscellaneous
C12N 9/1007
Transferring one-carbon groups: methyltransferases
C12N 9/80
Acting on carbon to nitrogen bonds other than peptide bonds: acting on amide bonds in linear amides