Compositions for genome editing
AssigneeEmendoBio Inc.
InventorsDavid Baram, Lior Izhar, Rafi Emmanuel
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Abstract
The present invention relates to compositions and methods for increasing the rate of site specific insertion of a donor DNA sequence to the genome. More specifically, the method introduces a donor DNA template containing at least one transcription factor binding site to a cell in order to favor specific insertion of a donor template sequence at a target site by homology directed repair (HDR).
Claims11
A composition comprising a donor DNA template molecule comprising a single stranded DNA (ssDNA) donor portion and a double stranded DNA (dsDNA) portion, wherein the dsDNA portion comprises at least one transcription factor binding site,
- wherein the transcription factor binding site is a binding site for a transcription factor selected from the group consisting of Sp1, TBP, TAFs, E2F, E-box and YY1;
- and
- wherein the ssDNA donor portion comprises
- a) a non-homologous sequence flanked by
- b) two regions homologous to a target gene.
A method for editing a genome in a cell comprising
- delivering to the cell a donor DNA template molecule comprising a single stranded DNA (ssDNA) donor portion and a double stranded DNA (dsDNA) portion, wherein the dsDNA portion comprises at least one transcription factor binding site,
- wherein the transcription factor binding site is a binding site for a transcription factor selected from the group consisting of Sp1, TBP, TAFs, E2F, E-box and YY1, and
- wherein the ssDNA donor portion comprises
- a) a non-homologous sequence flanked by
- b) two regions homologous to a target gene.
Dependencies are inferred from the claim text.