Type VI CRISPR orthologs and systems
AssigneesThe Broad Institute, Inc., Massachusetts Institute of Technology, President and Fellows of Harvard College
InventorsFeng Zhang, Omar O. Abudayyeh, Jonathan Gootenberg, Eric S. Lander
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Abstract
The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered RNA-targeting systems comprising a novel RNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA.
Claims24
A non-naturally occurring or engineered composition for modifying a target RNA sequence, said composition comprising:
- (a) a Cas13a having at least 95% sequence homology with Blautia sp. Marseille-P2398 (SEQ ID NO:
- 78), Thalassospira sp. TSL5-1 (SEQ ID NO:
- 83), SAMN04487830_13920 Pseudobutyrivibrio sp. OR37 (SEQ ID NO:
- 84), SAMN02910398_00008 Butyrivibrio sp. YAB3001 (SEQ ID NO:
- 85), Leptotrichia sp. Marseille-P3007 (SEQ ID NO:
- 87), Bacteroides ihuae (SEQ ID NO:
- 88), or Insolitispirillum peregrinum (SEQ ID NO:
- 91),
- (b) a guide molecule capable of forming a complex with the Cas13a and directing sequence-specific binding of the complex to a target sequence of a target RNA sequence, and
- (c) one or more heterologous functional domains associated with the Cas13a.
One or more polynucleotides encoding:
- (a) a Cas13a having at least 95% sequence homology with Blautia sp. Marseille-P2398 (SEQ ID NO:
- 78), Thalassospira sp. TSL5-1 (SEQ ID NO:
- 83), SAMN04487830_13920 Pseudobutyrivibrio sp. OR37 (SEQ ID NO:
- 84), SAMN02910398_00008 Butyrivibrio sp. YAB3001 (SEQ ID NO:
- 85), Leptotrichia sp. Marseille-P3007 (SEQ ID NO:
- 87), Bacteroides ihuae (SEQ ID NO:
- 88), or Insolitispirillum peregrinum (SEQ ID NO:
- 91),
- (b) a guide molecule capable of forming a complex with the Cas13a and directing sequence-specific binding of the complex to a target sequence of a target RNA sequence, and
- (c) one or more heterologous functional domains associated with the Cas13a.
A delivery system comprising a delivery vehicle, wherein the delivery vehicle is configured to deliver a non-naturally occurring or engineered composition comprising:
- (a) a Cas13a having at least 95% sequence homology with Blautia sp. Marseille-P2398 (SEQ ID NO:
- 78), Thalassospira sp. TSL5-1 (SEQ ID NO:
- 83), SAMN04487830_13920 Pseudobutyrivibrio sp. OR37 (SEQ ID NO:
- 84), SAMN02910398_00008 Butyrivibrio sp. YAB3001 (SEQ ID NO:
- 85), Leptotrichia sp. Marseille-P3007 (SEQ ID NO:
- 87), Bacteroides ihuae (SEQ ID NO:
- 88), or Insolitispirillum peregrinum (SEQ ID NO:
- 91),
- (b) a guide molecule capable of forming a complex with the Cas13a and directing sequence-specific binding of the complex to a target sequence of a target RNA sequence, and
- (c) one or more heterologous functional domains associated with the Cas13a.
Dependencies are inferred from the claim text.