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US 12,612,615 B2Granted
Isolated nucleic acid binding domains
OwnerArvydas Lubys, Algirdas Grybauskas +4inventors
InventorsArvydas Lubys, Algirdas Grybauskas, Dovile Strepetkaite, Zana Kapustina, Aliona Markina, Paulius Mielinis
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Abstract
Nucleic acid binding domains are described for use in isolating nucleic acid. Compositions and kits comprising these nucleic acid binding domains are also described. These nucleic acid binding domains may be used in a variety of methods.
Claims18
A method of isolating a nucleic acid from a sample, comprising:
- contacting one or more isolated nucleic acid binding domains with a sample comprising a nucleic acid under conditions suitable for binding, wherein the one or more isolated nucleic acid binding domains comprise SEQ ID NO:
- 10, SEQ ID NO:
- 15 with a mutation T10K, L16F, S20K, A27T, S41N, D44G, K55R, E54Q, D44Y, I52F, D45Q, S41R, E54A or T56I, or SEQ ID NO:
- 2 with a mutation R29D, and the one or more isolated nucleic acid binding domains are bound to a solid matrix either before or after contacting it with the sample;
- and
- separating the sample from the solid matrix with the bound one or more isolated nucleic acid binding domains bound to the nucleic acid.
3 more elements
Dependencies are inferred from the claim text.