Stabilization of RNA
AssigneeQiagen GmbH
InventorsMartin Horlitz, Uwe Oelmüller
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Abstract
The present invention pertains to methods, uses, compositions and kits for treating RNA at conditions comprising a temperature of at least 70° C. and a pH of at least 8, preferably a pH of at least 9. It also pertains to methods, uses and compositions for stabilizing RNA at conditions comprising a temperature of at least 70° C. and a pH of at least 8, preferably a pH of at least 9. It based on the finding that RNA can be stabilized in the presence of ammonium sulfate. In particular, the present invention provides a method of processing RNA, comprising treating the RNA at conditions comprising a temperature of at least 70° C. and a pH of at least 8, preferably a pH of at least 9, in the presence of ammonium sulfate at a concentration of 10 mM or less.
Claims18
A method of reducing, protecting from, inhibiting and/or preventing degradation of RNA, comprising treating the RNA at conditions comprising a temperature of at least 70° C. and a pH of at least 8 in the presence of ammonium sulfate, wherein
- ammonium sulfate is at a concentration of less than 10 mM,
- the RNA is bound to an anion exchange matrix, and
- the RNA is released from the anion exchange matrix during the treatment, thereby reducing, protecting from, inhibiting and/or preventing degradation of the RNA.
Dependencies are inferred from the claim text.