D-mannose isomerase and production method of D-fructose
AssigneeYamaguchi University
InventorsOsao Adachi, Toshiharu Yakushi, Naoya Kataoka, Kazunobu Matsushita
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Abstract
The present invention has an object to provide a D-mannose isomerase of membrane-bound form having the optimal reaction pH in acidic region. The membrane-bound D-mannose isomerase derived from acetic acid bacteria is produced. The acetic acid bacteria preferably belong to the genera of Acetobacter, Gluconobacter, or Gluconacetobacter. Furthermore, the membrane-bound D-mannose isomerase from the acetic acid bacteria is used to produce D-fructose from D-mannose.
Claims15
An isolated D-mannose isomerase bound to a cytoplasmic membrane of acetic acid bacteria,
- wherein the acetic acid bacteria belong to genera of Acetobacter, Gluconobacter, or Gluconacetobacter.
A method of producing an isolated D-mannose isomerase bound to a cytoplasmic membrane of acetic acid bacteria comprising:
- (a) culturing acetic acid bacteria in a nutrition medium to obtain bacterial cells;
- and
- (b) isolating the membrane-bound D-mannose isomerase from said bacterial cells by preparing a cytoplasmic membrane fraction from the bacterial cells, wherein the acetic acid bacteria belong to genera of Acetobacter, Gluconobacter, or Gluconacetobacter.
Dependencies are inferred from the claim text.