FnCpf1 mutant for broad-spectrum identification on PAM sequence ans use thereof
AssigneeInstitute of Microbiology, Chinese Academy of Sciences
InventorsZehua Chen, Ming Li
Life of this patent
Estimating the term…
Abstract
Provided is a mutant of CRISPR nuclease FnCpf1. Compared with wild-type FnCpf1, the CRISPR nuclease FnCpf1 has the following mutations: K671R/E566V/D751G/N508H/N637S, K671R/E566V/D751G/F570L/N634D/R755K, K671R/E566V/D751G/S518G/K639R, K671R/E566V/D751G/F570L/E686D, K671R/E566V/D751G/K613N/N637S/N534K/G664V, K671R/E566V/D751G/K613N/F570L/G664S/N637Y, K671R/E566V/D751G/K613N/Y724C/F570L, or K671R/E566V/D751G/K613N/Y724C/F570L/R690I/L662I. The coding gene of the mutant has higher editing efficiency and wider editing range than the wild-type FnCpf1.
Claims6
Mutants of CRISPR FnCpf1 or dFnCpf1 comprising the following mutations relative to wild-type FnCpf1 with the amino acid sequence shown in SEQ ID NO:2:
- K671R/E566V/D751G/N508H/N637S,
- K671R/E566V/D751G/F570L/N634D/R755K,
- K671R/E566V/D751G/S518G/K639R,
- K671R/E566V/D751G/F570L/E686D,
- K671R/E566V/D751G/K613N/N637S/N534K/G664V,
- K671R/E566V/D751G/K613N/F570L/G664S/N637Y,
- K671R/E566V/D751G/K613N/Y724C/F570L,
- K671R/E566V/D751G/K613N/Y724C/F570L/R690I/L662I.
A nucleic acid encoding a mutant CRISPR FnCpf1 or dFnCpf1, wherein the mutant CRISPR FnCpf1 or dFnCpf1 is one of the following mutations relative to wild-type FnCpf1 with the amino acid sequence in SEQ ID NO:
- 2:
- K671R/E566V/D751G/N508H/N637S,
- K671R/E566V/D751G/F570L/N634D/R755K,
- K671R/E566V/D751G/S518G/K639R, K671R/E566V/D751G/F570L/E686D,
- K671R/E566V/D751G/K613N/N637S/N534K/G664V,
- K671R/E566V/D751 G/K613N/F570L/G664S/N637Y,
- K671R/E566V/D751G/K613N/Y724C/F570L, and K671R/E566V/D751G/K613N/Y724C/F570L/R690I/L662I.
Dependencies are inferred from the claim text.