Use of cross-flow filtration device for preparing functional exosome

A method of preparing functional exosomes by modifying exosomes with functional materials using a tangential flow filtration (TFF) device,

• wherein the method comprises the following steps:
• a reaction step of circulating and mixing exosomes and functional materials inside the TFF device;
• a filtration and concentration step including removing unreacted functional materials;
• and
• a washing step of substituting a solvent of the mixed solution;
• wherein the reaction step is performed by allowing the exosomes and functional materials to circulate between a reservoir and a TFF filter inside the TFF device by blocking a tube connected to a permeate (P) direction of the TFF filter and a tube connected to the reservoir,
• wherein the filtration and concentration step and the washing step are performed simultaneously by opening both the tube connected to the P direction of the TFF filter and the tube connected to the reservoir,
• wherein the reaction step, the filtration and concentration step, and the washing step are performed using a first TFF device.