Method for forming reversible membraneless organelle in microorganisms

Abstract

The present invention provides a method for forming a reversible membraneless organelle in microorganisms. In the present invention, in order to form a membraneless organelle in a host cell, an artificial short peptide sequence WGR-1 is fused on the basis of an intrinsically disordered protein RGG. Fusion of one WGR-1 sequence or two WGR-1 sequences connected in series can both effectively promote phase separation of RGG, thus forming a membraneless organelle in Saccharomyces cerevisiae, wherein the membraneless organelle RGG-(WGR-1)2 has a better liquid fluidity. RGG-x-(WGR-1)2 is further constructed by introducing an enzyme cleavage site x into the RGG-(WGR-1)2 system in the present invention, and the reversible formation of the membraneless organelle is achieved after expression of a protease is induced. The method of the present invention has important value in customizing and controlling cell functions and the like.

Dependencies are inferred from the claim text.