Device that introduces substance to cells

A device for introducing a substance into cells in vitro, the device comprising:

• a housing;
• a syringe assembly detachably coupled to the housing, the syringe assembly comprising a syringe part, a plunger, a piston, an igniter, and a driving unit operatively coupled to each other,
• the syringe part coupled to the plunger,
• the plunger coupled to the piston,
• the piston coupled to the driving unit, and
• the igniter included in the driving unit;
• a button provided in the housing;
• a power source provided in the housing and coupled to the button, the power source configured to provide power to the igniter in response to the button being activated;
• an accommodation unit disposed between and coupled to the syringe part and the plunger and accommodating a solution containing the cells and the substance, the accommodation unit comprising:
• a nozzle, and
• an injection port disposed at a tip of the nozzle;
• the piston coupled with the accommodation unit via the plunger;
• the driving unit operatively coupled with the piston and the plunger, the driving unit causing a pressure in the accommodation unit to reach a maximum pressure in not longer than 2.0 msec from a pressurization start time for the solution by controlling the piston and the plunger to pressurize the solution accommodated in the accommodation unit through movement of the piston and the plunger;
• a sealing part sealing the injection port to prevent the solution from being injected from the accommodation unit to an outside of the syringe assembly via the injection port at least until the pressure in the accommodation unit reaches the maximum pressure;
• and
• a cap covering both the sealing part and a front portion of the syringe part that is aligned with the injection port of the nozzle, the cap fixing the sealing part to the front portion of the syringe part such that the sealing part is positioned between, and in contact with, the injection port and an inner surface of the cap.