US 12,612,448 B2 · Tumor-targeting A56 protein or fragment thereof, antibody binding to A56 protein, and use thereof

Abstract

A tumor-targeting protein or a fragment thereof, an antibody binding thereto and a use thereof are disclosed. A vector containing a nucleic acid coding for protein A56 or a fragment thereof, and a use thereof are disclosed. An antibody binding to protein A56 or a fragment thereof, and a use thereof are disclosed. The vector containing A56-encoding nucleic acid, a fragment thereof or a mutant thereof uses an oncolytic virus as the vector, and thus, when administered in an individual, specifically kills only cancer cells, primarily. In addition, cancer cells which have survived even after being infected with the oncolytic virus express the protein A56 on the cell surfaces thereof, and thus may be targeted for secondary anticancer therapy employing protein A56-encoding nucleic acid or protein A56 or a fragment thereof, or an antibody binding to A56.

An anticancer agent for targeting cancer cells infected with an oncolytic virus, comprising, as an active ingredient:

an antibody or an antigen-binding fragment thereof, which specifically binds to protein A56 or a fragment thereof,
wherein the oncolytic virus comprises a nucleic acid encoding the protein A56 or the fragment thereof, and
wherein the antibody is
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
1, a heavy chain CDR2 of SEQ ID NO:
2, a heavy chain CDR3 of SEQ ID NO:
3, a light chain CDR1 of SEQ ID NO:
4, a light chain CDR2 of SEQ ID NO:
5, and a light chain CDR3 of SEQ ID NO:
6;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
18, a heavy chain CDR2 of SEQ ID NO:
19, a heavy chain CDR3 of SEQ ID NO:
20, a light chain CDR1 of SEQ ID NO:
21, a light chain CDR2 of SEQ ID NO:
22, and a light chain CDR3 of SEQ ID NO:
23;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
35, a heavy chain CDR2 of SEQ ID NO:
36, a heavy chain CDR3 of SEQ ID NO:
37, a light chain CDR1 of SEQ ID NO:
38, a light chain CDR2 of SEQ ID NO:
39, and a light chain CDR3 of SEQ ID NO:
40;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
52, a heavy chain CDR2 of SEQ ID NO:
53, a heavy chain CDR3 of SEQ ID NO:
54, a light chain CDR1 of SEQ ID NO:
55, a light chain CDR2 of SEQ ID NO:
56, and a light chain CDR3 of SEQ ID NO:
57;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
69, a heavy chain CDR2 of SEQ ID NO:
70, a heavy chain CDR3 of SEQ ID NO:
71, a light chain CDR1 of SEQ ID NO:
72, a light chain CDR2 of SEQ ID NO:
73, and a light chain CDR3 of SEQ ID NO:
74;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
86, a heavy chain CDR2 of SEQ ID NO:
87, a heavy chain CDR3 of SEQ ID NO:
88, a light chain CDR1 of SEQ ID NO:
89, a light chain CDR2 of SEQ ID NO:
90, and a light chain CDR3 of SEQ ID NO:
91;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
103, a heavy chain CDR2 of SEQ ID NO:
104, a heavy chain CDR3 of SEQ ID NO:
105, a light chain CDR1 of SEQ ID NO:
106, a light chain CDR2 of SEQ ID NO:
107, and a light chain CDR3 of SEQ ID NO:
108;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
120, a heavy chain CDR2 of SEQ ID NO:
121, a heavy chain CDR3 of SEQ ID NO:
122, a light chain CDR1 of SEQ ID NO:
123, a light chain CDR2 of SEQ ID NO:
124, and a light chain CDR3 of SEQ ID NO:
125;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
137, a heavy chain CDR2 of SEQ ID NO:
138, a heavy chain CDR3 of SEQ ID NO:
139, a light chain CDR1 of SEQ ID NO:
140, a light chain CDR2 of SEQ ID NO:
141, and a light chain CDR3 of SEQ ID NO:
142;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
154, a heavy chain CDR2 of SEQ ID NO:
155, a heavy chain CDR3 of SEQ ID NO:
156, a light chain CDR1 of SEQ ID NO:
157, a light chain CDR2 of SEQ ID NO:
158, and a light chain CDR3 of SEQ ID NO:
159;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
171, a heavy chain CDR2 of SEQ ID NO:
172, a heavy chain CDR3 of SEQ ID NO:
173, a light chain CDR1 of SEQ ID NO:
174, a light chain CDR2 of SEQ ID NO:
175, and a light chain CDR3 of SEQ ID NO:
176;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
188, a heavy chain CDR2 of SEQ ID NO:
189, a heavy chain CDR3 of SEQ ID NO:
190, a light chain CDR1 of SEQ ID NO:
191, a light chain CDR2 of SEQ ID NO:
192, and a light chain CDR3 of SEQ ID NO:
193;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
205, a heavy chain CDR2 of SEQ ID NO:
206, a heavy chain CDR3 of SEQ ID NO:
207, a light chain CDR1 of SEQ ID NO:
208, a light chain CDR2 of SEQ ID NO:
209, and a light chain CDR3 of SEQ ID NO:
210;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
222, a heavy chain CDR2 of SEQ ID NO:
223, a heavy chain CDR3 of SEQ ID NO:
224, a light chain CDR1 of SEQ ID NO:
225, a light chain CDR2 of SEQ ID NO:
226, and a light chain CDR3 of SEQ ID NO:
227;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
239, a heavy chain CDR2 of SEQ ID NO:
240, a heavy chain CDR3 of SEQ ID NO:
241, a light chain CDR1 of SEQ ID NO:
242, a light chain CDR2 of SEQ ID NO:
243, and a light chain CDR3 of SEQ ID NO:
244;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
256, a heavy chain CDR2 of SEQ ID NO:
257, a heavy chain CDR3 of SEQ ID NO:
258 a light chain CDR1 of SEQ ID NO:
259, a light chain CDR2 of SEQ ID NO:
260, and a light chain CDR3 of SEQ ID NO:
261;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
273, a heavy chain CDR2 of SEQ ID NO:
274, a heavy chain CDR3 of SEQ ID NO:
275, a light chain CDR1 of SEQ ID NO:
276, a light chain CDR2 of SEQ ID NO:
277, and a light chain CDR3 of SEQ ID NO:
278;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
290, a heavy chain CDR2 of SEQ ID NO:
291, a heavy chain CDR3 of SEQ ID NO:
292, a light chain CDR1 of SEQ ID NO:
293, a light chain CDR2 of SEQ ID NO:
294, and a light chain CDR3 of SEQ ID NO:
295;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
307, a heavy chain CDR2 of SEQ ID NO:
308, a heavy chain CDR3 of SEQ ID NO:
309, a light chain CDR1 of SEQ ID NO:
310, a light chain CDR2 of SEQ ID NO:
311, and a light chain CDR3 of SEQ ID NO:
312;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
324, a heavy chain CDR2 of SEQ ID NO:
325, a heavy chain CDR3 of SEQ ID NO:
326, a light chain CDR1 of SEQ ID NO:
327, a light chain CDR2 of SEQ ID NO:
328, and a light chain CDR3 of SEQ ID NO:
329;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
341, a heavy chain CDR2 of SEQ ID NO:
342, a heavy chain CDR3 of SEQ ID NO:
343, a light chain CDR1 of SEQ ID NO:
344, a light chain CDR2 of SEQ ID NO:
345, and a light chain CDR3 of SEQ ID NO:
346;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
358, a heavy chain CDR2 of SEQ ID NO:
359, a heavy chain CDR3 of SEQ ID NO:
360, a light chain CDR1 of SEQ ID NO:
361, a light chain CDR2 of SEQ ID NO:
362, and a light chain CDR3 of SEQ ID NO:
363;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
375, a heavy chain CDR2 of SEQ ID NO:
376, a heavy chain CDR3 of SEQ ID NO:
377, a light chain CDR1 of SEQ ID NO:
378, a light chain CDR2 of SEQ ID NO:
379, and a light chain CDR3 of SEQ ID NO:
380;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
392, a heavy chain CDR2 of SEQ ID NO:
393, a heavy chain CDR3 of SEQ ID NO:
394, a light chain CDR1 of SEQ ID NO:
395, a light chain CDR2 of SEQ ID NO:
396, and a light chain CDR3 of SEQ ID NO:
397;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
409, a heavy chain CDR2 of SEQ ID NO:
410, a heavy chain CDR3 of SEQ ID NO:
411, a light chain CDR1 of SEQ ID NO:
412, a light chain CDR2 of SEQ ID NO:
413, and a light chain CDR3 of SEQ ID NO:
414;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
426, a heavy chain CDR2 of SEQ ID NO:
427, a heavy chain CDR3 of SEQ ID NO:
428, a light chain CDR1 of SEQ ID NO:
429, a light chain CDR2 of SEQ ID NO:
430, and a light chain CDR3 of SEQ ID NO:
431;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
443, a heavy chain CDR2 of SEQ ID NO:
444, a heavy chain CDR3 of SEQ ID NO:
445, a light chain CDR1 of SEQ ID NO:
446, a light chain CDR2 of SEQ ID NO:
447, and a light chain CDR3 of SEQ ID NO:
448;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
460, a heavy chain CDR2 of SEQ ID NO:
461, a heavy chain CDR3 of SEQ ID NO:
462, a light chain CDR1 of SEQ ID NO:
463, a light chain CDR2 of SEQ ID NO:
464, and a light chain CDR3 of SEQ ID NO:
465;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
477, a heavy chain CDR2 of SEQ ID NO:
478, a heavy chain CDR3 of SEQ ID NO:
479, a light chain CDR1 of SEQ ID NO:
480, a light chain CDR2 of SEQ ID NO:
481, and a light chain CDR3 of SEQ ID NO:
482;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
494, a heavy chain CDR2 of SEQ ID NO:
495, a heavy chain CDR3 of SEQ ID NO:
496, a light chain CDR1 of SEQ ID NO:
497, a light chain CDR2 of SEQ ID NO:
498, and a light chain CDR3 of SEQ ID NO:
499;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
511, a heavy chain CDR2 of SEQ ID NO:
512, a heavy chain CDR3 of SEQ ID NO:
513, a light chain CDR1 of SEQ ID NO:
514, a light chain CDR2 of SEQ ID NO:
515, and a light chain CDR3 of SEQ ID NO:
516;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
528, a heavy chain CDR2 of SEQ ID NO:
529, a heavy chain CDR3 of SEQ ID NO:
530, a light chain CDR1 of SEQ ID NO:
531, a light chain CDR2 of SEQ ID NO:
532, and a light chain CDR3 of SEQ ID NO:
533;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
545, a heavy chain CDR2 of SEQ ID NO:
546, a heavy chain CDR3 of SEQ ID NO:
547, a light chain CDR1 of SEQ ID NO:
548, a light chain CDR2 of SEQ ID NO:
549, and a light chain CDR3 of SEQ ID NO:
550;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
562, a heavy chain CDR2 of SEQ ID NO:
563, a heavy chain CDR3 of SEQ ID NO:
564, a light chain CDR1 of SEQ ID NO:
565, a light chain CDR2 of SEQ ID NO:
566, and a light chain CDR3 of SEQ ID NO:
567;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
579, a heavy chain CDR2 of SEQ ID NO:
580, a heavy chain CDR3 of SEQ ID NO:
581, a light chain CDR1 of SEQ ID NO:
582, a light chain CDR2 of SEQ ID NO:
583, and a light chain CDR3 of SEQ ID NO:
584;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
596, a heavy chain CDR2 of SEQ ID NO:
597, a heavy chain CDR3 of SEQ ID NO:
598, a light chain CDR1 of SEQ ID NO:
599, a light chain CDR2 of SEQ ID NO:
600, and a light chain CDR3 of SEQ ID NO:
601;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
613, a heavy chain CDR2 of SEQ ID NO:
614, a heavy chain CDR3 of SEQ ID NO:
615, a light chain CDR1 of SEQ ID NO:
616, a light chain CDR2 of SEQ ID NO:
617, and a light chain CDR3 of SEQ ID NO:
618;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
630, a heavy chain CDR2 of SEQ ID NO:
631, a heavy chain CDR3 of SEQ ID NO:
632, a light chain CDR1 of SEQ ID NO:
633, a light chain CDR2 of SEQ ID NO:
634, and a light chain CDR3 of SEQ ID NO:
635;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
647, a heavy chain CDR2 of SEQ ID NO:
648, a heavy chain CDR3 of SEQ ID NO:
649, a light chain CDR1 of SEQ ID NO:
650, a light chain CDR2 of SEQ ID NO:
651, and a light chain CDR3 of SEQ ID NO:
652;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
664, a heavy chain CDR2 of SEQ ID NO:
665, a heavy chain CDR3 of SEQ ID NO:
666, a light chain CDR1 of SEQ ID NO:
667, a light chain CDR2 of SEQ ID NO:
668, and a light chain CDR3 of SEQ ID NO:
669;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
681, a heavy chain CDR2 of SEQ ID NO:
682, a heavy chain CDR3 of SEQ ID NO:
683, a light chain CDR1 of SEQ ID NO:
684, a light chain CDR2 of SEQ ID NO:
685, and a light chain CDR3 of SEQ ID NO:
686;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
698, a heavy chain CDR2 of SEQ ID NO:
699, a heavy chain CDR3 of SEQ ID NO:
700, a light chain CDR1 of SEQ ID NO:
701, a light chain CDR2 of SEQ ID NO:
702, and a light chain CDR3 of SEQ ID NO:
703;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
715, a heavy chain CDR2 of SEQ ID NO:
716, a heavy chain CDR3 of SEQ ID NO:
717, a light chain CDR1 of SEQ ID NO:
718, a light chain CDR2 of SEQ ID NO:
719, and a light chain CDR3 of SEQ ID NO:
720;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
732, a heavy chain CDR2 of SEQ ID NO:
733, a heavy chain CDR3 of SEQ ID NO:
734, a light chain CDR1 of SEQ ID NO:
735, a light chain CDR2 of SEQ ID NO:
736, and a light chain CDR3 of SEQ ID NO:
737;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
749, a heavy chain CDR2 of SEQ ID NO:
750, a heavy chain CDR3 of SEQ ID NO:
751, a light chain CDR1 of SEQ ID NO:
752, a light chain CDR2 of SEQ ID NO:
753, and a light chain CDR3 of SEQ ID NO:
754;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
766, a heavy chain CDR2 of SEQ ID NO:
767, a heavy chain CDR3 of SEQ ID NO:
768, a light chain CDR1 of SEQ ID NO:
769, a light chain CDR2 of SEQ ID NO:
770, and a light chain CDR3 of SEQ ID NO:
771;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
783, a heavy chain CDR2 of SEQ ID NO:
784, a heavy chain CDR3 of SEQ ID NO:
785, a light chain CDR1 of SEQ ID NO:
786, a light chain CDR2 of SEQ ID NO:
787, and a light chain CDR3 of SEQ ID NO:
788;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
800, a heavy chain CDR2 of SEQ ID NO:
801, a heavy chain CDR3 of SEQ ID NO:
802, a light chain CDR1 of SEQ ID NO:
803, a light chain CDR2 of SEQ ID NO:
804, and a light chain CDR3 of SEQ ID NO:
805;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
817, a heavy chain CDR2 of SEQ ID NO:
818, a heavy chain CDR3 of SEQ ID NO:
819, a light chain CDR1 of SEQ ID NO:
820, a light chain CDR2 of SEQ ID NO:
821, and a light chain CDR3 of SEQ ID NO:
822;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
834, a heavy chain CDR2 of SEQ ID NO:
835, a heavy chain CDR3 of SEQ ID NO:
836, a light chain CDR1 of SEQ ID NO:
837, a light chain CDR2 of SEQ ID NO:
838, and a light chain CDR3 of SEQ ID NO:
839;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
851, a heavy chain CDR2 of SEQ ID NO:
852, a heavy chain CDR3 of SEQ ID NO:
853, a light chain CDR1 of SEQ ID NO:
854, a light chain CDR2 of SEQ ID NO:
855, and a light chain CDR3 of SEQ ID NO:
856;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
868, a heavy chain CDR2 of SEQ ID NO:
869, a heavy chain CDR3 of SEQ ID NO:
870, a light chain CDR1 of SEQ ID NO:
871, a light chain CDR2 of SEQ ID NO:
872, and a light chain CDR3 of SEQ ID NO:
873;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
885, a heavy chain CDR2 of SEQ ID NO:
886, a heavy chain CDR3 of SEQ ID NO:
887, a light chain CDR1 of SEQ ID NO:
888, a light chain CDR2 of SEQ ID NO:
889, and a light chain CDR3 of SEQ ID NO:
890;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
902, a heavy chain CDR2 of SEQ ID NO:
903, a heavy chain CDR3 of SEQ ID NO:
904, a light chain CDR1 of SEQ ID NO:
905, a light chain CDR2 of SEQ ID NO:
906, and a light chain CDR3 of SEQ ID NO:
907;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
919, a heavy chain CDR2 of SEQ ID NO:
920, a heavy chain CDR3 of SEQ ID NO:
921, a light chain CDR1 of SEQ ID NO:
922, a light chain CDR2 of SEQ ID NO:
923, and a light chain CDR3 of SEQ ID NO:
924;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
936, a heavy chain CDR2 of SEQ ID NO:
937, a heavy chain CDR3 of SEQ ID NO:
938, a light chain CDR1 of SEQ ID NO:
939, a light chain CDR2 of SEQ ID NO:
940, and a light chain CDR3 of SEQ ID NO:
941;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
953, a heavy chain CDR2 of SEQ ID NO:
954, a heavy chain CDR3 of SEQ ID NO:
955, a light chain CDR1 of SEQ ID NO:
956, a light chain CDR2 of SEQ ID NO:
957, and a light chain CDR3 of SEQ ID NO:
958;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
970, a heavy chain CDR2 of SEQ ID NO:
971, a heavy chain CDR3 of SEQ ID NO:
972, a light chain CDR1 of SEQ ID NO:
973, a light chain CDR2 of SEQ ID NO:
974, and a light chain CDR3 of SEQ ID NO:
975;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
987, a heavy chain CDR2 of SEQ ID NO:
988, a heavy chain CDR3 of SEQ ID NO:
989, a light chain CDR1 of SEQ ID NO:
990, a light chain CDR2 of SEQ ID NO:
991, and a light chain CDR3 of SEQ ID NO:
992;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
1004, a heavy chain CDR2 of SEQ ID NO:
1005, a heavy chain CDR3 of SEQ ID NO:
1006, a light chain CDR1 of SEQ ID NO:
1007, a light chain CDR2 of SEQ ID NO:
1008, and a light chain CDR3 of SEQ ID NO:
1009;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
1021, a heavy chain CDR2 of SEQ ID NO:
1022, a heavy chain CDR3 of SEQ ID NO:
1023, a light chain CDR1 of SEQ ID NO:
1024, a light chain CDR2 of SEQ ID NO:
1025, and a light chain CDR3 of SEQ ID NO:
1026;
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
1062, a heavy chain CDR2 of SEQ ID NO:
1064, a heavy chain CDR3 of SEQ ID NO:
1065, a light chain CDR1 of SEQ ID NO:
1066, a light chain CDR2 of SEQ ID NO:
1067, and a light chain CDR3 of SEQ ID NO:
1068;
or
an antibody comprising a heavy chain CDR1 of SEQ ID NO:
1063, a heavy chain CDR2 of SEQ ID NO:
1064, a heavy chain CDR3 of SEQ ID NO:
1065, a light chain CDR1 of SEQ ID NO:
1066, a light chain CDR2 of SEQ ID NO:
1067, and a light chain CDR3 of SEQ ID NO:
1068.

442 more elements

Tumor-targeting A56 protein or fragment thereof, antibody binding to A56 protein, and use thereof

Life of this patent

Tumor-targeting A56 protein or fragment thereof, antibody binding to A56 protein, and use thereof

Life of this patent

Abstract

Claims6