T cell receptors targeting mutations in RNA splicing factors
AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorsUdai Shankar Kammula, Ghanshyam Singh Yadav, Chetana Bhaskarla
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Abstract
This document provides isolated immune cells that include an exogenous T cell receptor (TCR) having affinity for a splicing factor 3B subunit 1A (SF3B1) peptide as well as methods and materials for making such immune cells. For example, isolated immune cells that included an exogenous TCR having affinity for a mutant SF3B1 peptide, methods and materials for making such immune cells, and methods and materials for using such immune cells to treat mammals (e.g., a human having cancer) are provided.
Claims22
An isolated human immune cell comprising an exogenous T cell receptor (TCR) having affinity for a human splicing factor 3B subunit 1A (SF3B1) peptide comprising a histidine at position 625 in the context of DRB3, said exogenous TCR comprising an alpha chain and a beta chain,
- said alpha chain comprising the amino acid sequences set forth in SEQ ID NO:10, SEQ ID NO:11, and SEQ ID NO:12, and
- said beta chain comprising the amino acid sequences set forth in SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3.
A nucleic acid molecule comprising (i) a nucleic acid sequence encoding an alpha chain of a TCR having affinity for a human SF3B1 peptide comprising a histidine at position 625 in the context of DRB3 and (ii) a nucleic acid sequence encoding a beta chain of said TCR,
- said alpha chain comprising the amino acid sequences set forth in SEQ ID NO:10, SEQ ID NO:11, and SEQ ID NO:12, and
- said beta chain comprising the amino acid sequences set forth in SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3.
Dependencies are inferred from the claim text.