Mutant bacteriorhodopsin-like-channelrhodopsin ion channel
AssigneesGeorg-August-UniversitäT GöTtingen Stiftung öFfentlichen Rechts, UniversitäTsmedizin, OptoGenTech GmbH
InventorsTobias Moser, Thomas Mager, Maria Zerche
Life of this patent
Estimating the term…
Abstract
The present invention relates to a mutant ion channel capable of being activated by light (‘light-activated’ ion channel) and having improved properties, nucleic acids and expression vectors encoding the mutant ion channel, cells comprising such nucleic acid or expression vector, devices containing the mutant ion channel, nucleic acid or expression vector as well as respective uses and methods.
Claims14
A mutant ion channel, wherein the mutant ion channel comprises a 7-transmembrane-helix motif having an amino acid sequence identical with the full-length sequence of the 7-transmembrane-helix motif of the wild-type ion channel RICCR1 set forth in SEQ ID NO:9, except for one or both of:
- (i) Leu, Ile or Val at the amino acid position corresponding to T218 in SEQ ID NO:5;
- (ii) Ala, Gly or Val at the amino acid position corresponding to S220 in SEQ ID NO:5;
- and, optionally, one or more of:
- (iii) Phe at the amino acid position corresponding to Y260 in SEQ ID NO:5,
- (iv) His at the amino acid position corresponding to R136 in SEQ ID NO:5,
- (v) Trp at the amino acid position corresponding to S138 in SEQ ID NO:5,
- (vi) Phe at the amino acid position corresponding to Y156 in SEQ ID NO:5,
- (vii) Phe at the amino acid position corresponding to Y116 in SEQ ID NO:5,
- (viii) Val at the amino acid position corresponding to T119 in SEQ ID NO:5;
- and
- wherein the mutant ion channel is capable of being activated by light;
- and
- wherein if there is Ala at the amino acid position corresponding to S220 in SEQ ID NO:5, there is Leu, Ile or Val at the amino acid position corresponding to T218 in SEQ ID NO:5.
Dependencies are inferred from the claim text.