Zein-enriched and depleted protein
AssigneeCargill, Incorporated
InventorsErika Lyn Mcconville, Michael A. Porter
Life of this patent
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Abstract
Described herein is a corn protein product, comprising a first fraction comprising 75 wt % to 95 wt % (dry solids) protein and a second fraction comprising 60 wt % to 80 wt % (dry solids) protein, wherein the first fraction is a zein-enriched fraction and the second fraction is a zein-depleted fraction and a method of achieving the same. Further described herein is a corn protein product derived from destarched corn gluten meal comprising a first fraction comprising 78 wt % to 83 wt % (dry solids) protein and a second fraction comprising 70 wt % to 80 wt % (dry solids) protein, wherein the first fraction is a zein-enriched fraction and the second fraction is a zein-depleted fraction.
Claims15
A method of obtaining a corn protein zein-enriched fraction product and a corn protein zein-depleted fraction product, comprising
- obtaining an enzymatically destarched corn gluten meal that has been prepared by a process consisting of
- providing corn gluten meal,
- destarching the corn gluten meal by enzymatic treatment,
- optionally rinsing with water,
- optionally defatting using solvent or enzymes,
- optionally decolorizing by bleaching with enzymatic methods or chemical bleaching agents selected from ozone, persulfate and peroxides, and
- optionally filtering;
- and
- carrying out an extraction step by treating the thus obtained enzymatically destarched corn gluten meal with an ethanol-water solvent comprising 55-80 wt % ethanol without an intervening step to obtain
- a) a corn protein zein-enriched fraction product comprising 75 wt % to 95 wt % (dry solids) protein, and
- b) a corn protein zein-depleted fraction product comprising 60 wt % to 80 wt % (dry solids) protein;
- wherein
- the corn protein zein-enriched fraction product is enriched in aspartic acid, isoleucine and lysine as compared to a comparison corn protein zein-enriched fraction product that has been prepared from corn gluten meal that has not been enzymatically destarched, and
- the corn protein zein-enriched fraction product is depleted in glycine as compared to a comparison corn protein zein-enriched fraction product that has been prepared from corn gluten meal that has not been enzymatically destarched;
- and
- wherein
- the corn protein zein-depleted fraction product is enriched in leucine and tyrosine as compared to a comparison corn protein zein-enriched fraction product that has been prepared from corn gluten meal that has not been enzymatically destarched, and
- the corn protein zein-depleted fraction product is depleted in ammonium chloride, arginine, aspartic acid and lysine as compared to a comparison corn protein zein-enriched fraction product that has been prepared from corn gluten meal that has not been enzymatically destarched.
Dependencies are inferred from the claim text.