Variants of hlya and uses thereof
AssigneeNumaferm GmbH
InventorChristian Schwarz
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Abstract
Polypeptides comprising hemolysin A (Hly A) fragments and/or variants and a peptide or polypeptide of interest in form of a fusion protein that exhibits improved expression and secretion as well as increased solubility and stability and improved renaturation efficiency. Also encompassed are nucleic acids encoding these polypeptides. host cells that comprise said nucleic acids, and methods and uses for protein expression using said nucleic acids, host cells and polypeptides.
Claims15
A polypeptide variant comprising a first amino acid sequence, wherein the first amino acid sequence has at least 80% sequence identity over its entire length with the amino acid sequence set forth in SEQ ID NO:1 (HlyA1) and comprises at least one amino acid substitution or deletion at the position corresponding to position 198 of SEQ ID NO:1, and wherein the first amino acid sequence is at least 100 amino acids in length.
Dependencies are inferred from the claim text.