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US 12,611,666 B2Granted
Substrate with orthogonally functional nanodomains
AssigneeIllumina, Inc.
InventorsAlexandra Szemjonov, Wayne N. George, Alexandre Richez
Life of this patent
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Abstract
Embodiments of the present disclosure also relate to methods of fabricating flow cell substrates. Some exemplary workflows exploit orthogonal chemistries of substrate layers such that the process does not include polishing steps. Substrates prepared by the method described herein can include a first primer set and a second primer set compatible with simultaneous paired-end sequencing methods.
Claims22
A patterned substrate, comprising:
- a base support;
- a multi-layer stack positioned over the base support;
- the multi-layer stack comprising:
- a first layer positioned over the base support;
- a second layer positioned over the first layer, wherein the second layer comprises a plurality of depressions through the second layer and expose a surface of the first layer;
- and
- a functionalized hydrogel within at least a portion of the plurality of depressions, wherein the functionalized hydrogel is covalently attached to the first layer;
- wherein the first layer comprises poly(allylamine) (PAAm) and the second layer comprises poly (propargyl methacrylate) (PPMA), or the first layer comprises PPMA and the second layer comprises PAAm.
5 more elements
A patterned substrate, comprising:
- a base support;
- a multi-layer stack positioned over the base support;
- the multi-layer stack comprising:
- a first layer positioned over the base support;
- a second layer positioned over the first layer, wherein the second layer comprises a first plurality of depressions through the second layer which expose a surface of the first layer;
- and
- a third layer positioned over the second layer, wherein the third layer comprises a second plurality of depressions through the third layer which expose a surface of the second layer, wherein the second plurality of depressions are spatially separated from the first plurality of depressions;
- a first functionalized hydrogel within at least a portion of the first plurality of depressions, wherein the first functionalized hydrogel is covalently attached to the first layer;
- and
- a second functionalized hydrogel within at least a portion of the second plurality of depressions, wherein the second functionalized hydrogel is covalently attached to the second layer;
- wherein the first layer of the multi-layer stack comprises a first polymer having a plurality of first functional groups selected from the group consisting of an amino, an alkynyl, a cycloalkenyl, a cycloalkynyl, a carboxyl, a vinyl, and a thiol;
- wherein the second layer of the multi-layer stack comprises a second polymer having a plurality of second functional groups selected from the group consisting of an amino, an alkynyl, a cycloalkenyl, a cycloalkynyl, a carboxyl, a vinyl, and a thiol, and the second functional groups are orthogonal to the first functional groups;
- and
- wherein the third layer does not comprise either the first or the second functional groups, and wherein the third layer is inert to both the first functionalized hydrogel and the second functionalized hydrogel.
11 more elements
Dependencies are inferred from the claim text.