Method and kit for preservation of adipose tissue grafts

A method of preparing a fat graft comprising:

• introducing fat tissue into an internal chamber of a vessel of at least one cell or tissue isolation, purification, and storage device through a first opening of the vessel disposed at a first end of the at least one cell or tissue isolation, purification, and storage device;
• introducing an amount of a cryoprotectant effective to cryopreserve the fat graft into the internal chamber of the vessel through a second opening of the vessel disposed at a second end of the at least one cell or tissue isolation, purification, and storage device;
• mixing the fat tissue with the cryoprotectant;
• when the cryoprotectant is introduced, cooling the fat graft to a temperature below 0° C.;
• and
• storing the fat graft in the vessel of the at least one cell or tissue isolation, purification, and storage device at the temperature of below 0° C. until the fat graft is to be used for delivery to a patient.