Methods for producing transplantable cartilage tissue
AssigneeCo.Don AG
InventorsGiulietta Roël, Christian Kaps, Claudia Eschen
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Abstract
The present invention relates to a method for producing cartilage tissue by selecting and choosing from chondrocytes or spheroids, and use thereof as pharmaceutical composition, medicinal product or transplant. In particular, the invention relates to markers for identifying suitable chondrocytes for producing cartilage tissue, in particular for the purpose of transplantation.
Claims8
A method for selecting chondrocytes for production of transplantable cartilage tissue, the method comprising:
- multiplying the chondrocytes in a monolayer culture;
- determining gene expression levels of anosmin 1 (KAL1) in the multiplied chondrocytes in the monolayer culture;
- selecting multiplied chondrocytes that express KAL1;
- aggregating the selected multiplied chondrocytes to form spheroids;
- determining gene expression levels of cartilage acidic protein 1 (CRTAC1) and early B-cell factor 3 (EBF3), and at least one of neuritin 1 (NRN1) and aggrecan (ACAN) in the spheroids;
- and
- selecting spheroids that express CRTAC1 and at least one of NRN1 and ACAN but have expression levels of EBF3 below a predetermined threshold value for the production of the transplantable cartilage tissue.
A method for selecting chondrocytes for the production of transplantable cartilage tissue, the method comprising:
- multiplying the chondrocytes in a monolayer culture;
- determining gene expression levels of KAL1 in the multiplied chondrocytes;
- selecting multiplied chondrocytes that express KAL1;
- aggregating the selected multiplied chondrocytes to form spheroids;
- determining gene expression levels of cartilage acidic protein 1 (CRTAC1), neuritin 1 (NRN1), aggrecan (ACAN), and early B-cell factor 3 (EBF3) in the spheroids;
- and
- selecting spheroids that express CRTAC1, NRN1, and ACAN but have expression levels of EBF3 below a predetermined threshold value for the production of the transplantable cartilage tissue.
A method for selecting chondrocytes for the production of transplantable cartilage tissue, the method comprising:
- multiplying the chondrocytes in a monolayer culture;
- determining gene expression levels of KAL1 in the multiplied chondrocytes;
- selecting multiplied chondrocytes that express KAL1;
- and
- aggregating the selected multiplied chondrocytes to form spheroids;
- determining gene expression levels of cartilage acidic protein 1 (CRTAC1), aggrecan (ACAN), and early B-cell factor 3 (EBF3) in the spheroids;
- and
- selecting spheroids that express CRTAC1 and ACAN but have expression levels of EBF3 below a predetermined threshold value for the production of the transplantable cartilage tissue.
Dependencies are inferred from the claim text.