US 12,611,485 B2 · Composite demineralized bone matrix composition containing bone mineral component and method for producing same

Abstract

A method for producing a composite demineralized bone matrix composition using a one-step process is described. The composite demineralized bone matrix composition is produced from the biologically-derived bone. In addition, the composite demineralized bone matrix composition contains bone minerals according to the original composition proportion in the bone and may provide a bone mineral content condition that is closest to that in an environment in which in vivo bone formation occurs. In addition, the composition contains a bone morphogenetic protein (BMP-2), and thus enables a stable and excellent bone formation effect to be derived.

Claims7

Dependent claims

A method of preparing a composite demineralized bone matrix (DBM) composition using a one-step process, comprising:

a demineralization step of demineralizing bone tissue with an acidic solution;
a bone mineral precipitation step of precipitating bone mineral by neutralizing the demineralized solution with an alkaline solution;
a bone morphogenetic protein-2 (BMP-2) extraction step of extracting BMP-2 by centrifuging the solution in which the precipitation is completed and adding an acidic solution to a pellet;
and
a composite DBM composition preparation step of precipitating the DBM, the bone mineral, and the BMP-2 by neutralizing the BMP-2-extracted solution with an alkaline solution.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

17/772,849Filed 22 May 2020

Priority

KR 10-2019-013647730 Oct 2019

Published

US 2022/0378980 A11 Dec 2022

Granted

28 Apr 2026

Term ends

Not estimatedThe source records don’t support an estimate.

Classification20 codes

A61L 27/3608
Characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura,…
A61F 2/28
Prostheses implantable into the body: bones
A61L 27/26
Macromolecular materials: mixtures of macromolecular compounds
A61L 27/365
Connective tissue: bones

Composite demineralized bone matrix composition containing bone mineral component and method for producing same

Life of this patent

Composite demineralized bone matrix composition containing bone mineral component and method for producing same

Life of this patent

Abstract

Claims7