Tomato plant having improved insect resistance
AssigneeEnza Zaden Beheer B.V.
InventorsTeresa Maria Montoro Ponsoda, Jan-Willem De Kraker, Ilona Gertruida Maria Broersen, Ilja Roobeek, Marieke Ykema, Fausto Rodriguez Sánchez
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Abstract
The present invention relates to a tomato plant having improved insect resistance, more specifically whitefly or mite resistance, wherein the plant comprises a SlAT2 gene encoding for an acetyl-CoA-dependent acyltransferase enzyme and an AP2e gene encoding for a APETALA2 ethylene-responsive transcription factor. The present invention further relates to methods for providing a tomato plant having improved insect resistance and the use of a SlAT2 gene in combination with an AP2e gene for providing insect resistant tomato plants.
Claims17
A tomato plant having improved whitefly resistance, wherein said plant comprises a combination of an acetyl-CoA-dependent acyltransferase gene (SlAT2) encoding a cDNA sequence having at least 99% sequence identity with SEQ ID No. 2, and an APETALA2e ethylene-responsive transcription factor gene (AP2e) encoding a cDNA sequence of SEQ ID No. 5, wherein said combination of SlAT2 and AP2e genes result in an increased C29H48O15 and C36H62O15 acyl sugar content as compared to a tomato plant not comprising said combination of genes.
A method for providing a tomato plant having improved whitefly resistance comprising the steps of:
- providing a whitefly susceptible tomato plant;
- and
- mutating its genome by introducing therein a combination of an acetyl-CoA-dependent acyltransferase gene (SlAT2) encoding a cDNA sequence-having at least 99% sequence identity with SEQ ID No. 2, and an APETALA2e ethylene-responsive transcription factor gene (AP2e) encoding a cDNA sequence of SEQ ID No. 5, wherein said combination of SlAT2 and AP2e genes result in an increased C29H40s15 and C36H62O is acyl sugar content as compared to a tomato plant not comprising said combination of genes.
A composition comprising a combination of two genomic regions for providing insect resistance in tomato plants, wherein one genomic region comprising SEQ ID No. 1 that encodes an acetyl-CoA-dependent acyltransferase gene (SlAT2) and a second genomic region comprising SEQ ID No. 4 that encodes an APETALA2e ethylene-responsive transcription factor gene (AP2e).
A composition comprising a combination of two genes for providing insect resistance in tomato plants, wherein one gene encodes an acetyl-CoA-dependent acyltransferase (SlAT2) protein comprising SEQ ID No. 3 and a second gene that encodes an APETALA2e ethylene-responsive transcription factor (AP2e) comprising SEQ ID No. 6.
Dependencies are inferred from the claim text.