Microscope automatic focusing method, microscope system, medical device, and storage medium

A medical device, comprising:

• a microscope system, a memory, and a processor, the microscope system comprising:
• an objective lens, configured to gather a first light of a target sample to enter a first optical path, wherein the first light converges, at a beamsplitter, with a second light generated by an image projection module after entering the first optical path through a lens assembly;
• a beamsplitter assembly, comprising the beamsplitter and configured to respectively separate and cast lights in different optical paths;
• a camera assembly, disposed in the first optical path, comprising a camera, and configured to photograph the target sample in a microscope field of view, to generate and output a focused image photographed through the first optical path;
• an auxiliary focusing device, comprising an auxiliary focusing light source and an auxiliary focusing camera, disposed in a second optical path, and configured to determine a focal length matching the camera assembly;
• and
• a focusing device, configured to adjust a focal length of image light entering the camera assembly according to a defocus amount of a target sample image determined by the auxiliary focusing device;
• the memory being configured to store executable instructions;
• and
• the processor being configured to perform:
• obtaining a measurement sample photographed by using the auxiliary focusing camera in the second optical path;
• calculating an image evaluation parameter according to the measurement sample photographed by the auxiliary focusing camera and an image evaluation standard corresponding to the measurement sample;
• searching a prestored calibration curve for a relationship between the image evaluation parameter and a defocus amount according to the image evaluation parameter, to determine a required defocus amount;
• and
• adjusting a focal length of the image light entering the camera assembly according to the determined defocus amount, to photograph a clearly focused image by using the camera assembly through the first optical path.