Super-resolution morphology-correlated detection of labile zinc
AssigneeUniversity of Cincinnati
InventorJiajie Diao
Life of this patent
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Abstract
A method for detecting labile zinc (Zn2+) in a biological material is provided herein, the method including: (a) contacting the biological material with a composition including NapBu-BPEA; and (b) imaging the biological material via molecular fluorescence imaging to detect the labile zinc in the biological material. Also provided herein are methods for morphology-correlated detection of labile zinc localization in a subcellular organelle of a living cell and methods for tracking a change in labile zinc localization in a biological material.
Claims23
A method for detecting labile zinc (Zn2+) in a biological material, the method comprising:
- (a) contacting the biological material with a composition comprising 2,4-(bis(pyridin-2-ylmethyl) aminoethyl) amino-N-n-butyl-1,8-naphthalimide (NapBu-BPEA), having the structure
- wherein the NapBu-BPEA binds to the labile zinc and fluoresces upon excitation;
- and
- (b) imaging the biological material via molecular fluorescence imaging to detect the labile zinc in the biological material.
A method for morphology-correlated detection of labile zinc (Zn2+) localization in a subcellular organelle of a living cell, the method comprising:
- (a) contacting the cell with a composition comprising 2,4-(bis(pyridin-2-ylmethyl) aminoethyl) amino-N-n-butyl-1,8-naphthalimide (NapBu-BPEA), wherein the NapBu-BPEA binds the labile zinc and fluoresces upon excitation;
- (b) imaging the cell via super-resolution imaging to visualize a distribution of labile zinc in the cell;
- and
- (c) correlating the distribution of labile zinc in the cell with at least one morphological feature of the subcellular organelle to determine labile zinc localization in the subcellular organelle.
Dependencies are inferred from the claim text.