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US 12,607,636 B2Granted
Carbene mass tagging
AssigneeNortheastern University
InventorsPushkar Kulkarni, Roger W. Giese, Poguang Wang
Life of this patent
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Abstract
The disclosure relates to a diazirine precursor mass tag compound represented by structural formula (I) Also disclosed is a method for detecting analytes in a sample, comprising derivatizing the analytes with the compound of formula (I), and detecting the resulting derivatized analytes by a mass or ion mobility spectrometry.
Claims20
A compound consisting of an R1-aryl1—CHR2—NR3R4R5 (RACN) moiety, a single polyfluoroalkyldiazirine (PFAD) group, and a counterion;
- wherein:
- R1 is H, XCH3, XCH2CH3, CH2XCH3, CH2XCH2CH3, or a group that connects to the PFAD group;
- X is O or S;
- aryl is phenyl, pyridyl, or pyrimidyl;
- R2 is H or a group that connects to the PFAD group;
- R3 is H or an alkyl or aryl group having 6 or fewer carbon atoms;
- each of R4 and R5 is an alkyl or aryl group having 6 or fewer carbon atoms;
- and
- the counterion is selected from the group consisting of bromide, chloride, fluoride, nitrate, acetate, trifluoroacetate, pivalate, bicarbonate, arsenite, dihydrogenarsenate, hexafluoroarsenate, perfluorobutane sulfonate, perfluorohexane sulfonate, perfluorooctane sulfonate, perchlorate, cyanide, tetrafluoroborate, trichlorostannate, trifluorosulfonate, and pentafluorophenolate.
6 more elements
A compound represented by structural formula (I)
- wherein, independently for each occurrence,
- Q is —(NR6R7R8)+X−;
- X is halide, NO3, OC(O)CH3, OC(O)C(CH3)3, OC(O)CF3, HCO3, AsO2, H2AsO4, AsF6, SO3(C4F9), SO3 (C6F13), SO3C8F17, ClO4, CN, BF4, SnC13, CF3SO3, or C6F5O;
- R6, R7, R8 is each independently C1-6 alkyl or C6aryl;
- Y is phenyl, pyridyl, or pyrimidyl;
- Z is —(CH2)A(CH2)k—;
- L is —(CH2)A(CH2)k— or absent;
- A is O, S, or NH;
- 1 is 0 to 2;
- k is 0 to 2;
- m is 0 to 10;
- and
- n is 1 to 3.
10 more elements
A compound selected from
Dependencies are inferred from the claim text.