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US 12,607,635 B2Granted
Method of labelling viral particles comprising anionic lipids with fluorescently-labelled negatively-charged polynucleotides in the presence of polyvalent cations
AssigneeOxford University Innovation Limited
InventorsNicole Robb, Achillefs Kapanidis, Jonathan Taylor
Life of this patent
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Abstract
Provided herein is a method of functionalizing a particle, as well as methods of optically tracking a particle, isolating enveloped viral particles from a sample, quantifying enveloped virus particles in a sample and assessing enveloped viral aggregation in a sample. Kits are also provided. The particle is typically a viral particle.
Claims17
A method of labelling a particle having an anionic lipid bilayer with a fluorescently labelled negatively charged non-specific polynucleotide, comprising:
- contacting a particle having an anionic lipid bilayer with a fluorescently labelled negatively charged non-specific polynucleotide in the presence of a polyvalent cation, thereby creating a labelled particle.
The method of 1 wherein the particle is an enveloped virus particle.
Dependencies are inferred from the claim text.