Nanoparticle solutions, kits, devices and methods of use thereof
AssigneeAgency for Science, Technology and Research
InventorsLaura Sutarlie, Xiaodi Su, Yang Xu, Heng Li Chee, Sian Yang Ow, Takuya Sato
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Abstract
The present invention relates, in general terms, to nanoparticle solutions, kits, devices and methods of use thereof. The present invention is suitable for use in quantifying bacterial cells in a sample. The method of quantifying bacterial cells in a sample comprises passing the sample in a liquid form and an aqueous nanoparticle solution through a porous substrate such that the bacterial cells in the sample is trapped on the porous substrate and can be quantified by a colorimetric and/or fluorescence output emitted from the nanoparticle bound to the bacterial cells.
Claims20
A method of quantifying bacterial cells in a sample, comprising:
- a) passing the sample in a liquid form through a porous substrate, the porous substrate for trapping or retaining bacterial cells on its surface thereof;
- b) passing an aqueous nanoparticle solution through the porous substrate, the aqueous nanoparticle solution comprising a plasmonic and/or fluorescent nanoparticle and a first ionic surfactant, the nanoparticle functionalised with an affinity probe for binding to said bacterial cells trapped or retained on the surface of the substrate via affinity binding;
- and
- c) passing a second aqueous solution through the porous substrate, the second aqueous solution for washing the unbound nanoparticle from the porous substrate;
- wherein the bacterial cells in the sample are quantifiable by a colorimetric and/or fluorescence output emitted from the nanoparticle bound to the bacterial cells.
A device for quantifying bacterial cells in a sample in a liquid form, comprising:
- a) a porous substrate for trapping or retaining the bacterial cells on its surface thereof;
- and
- b) an aqueous nanoparticle solution comprising a plasmonic and/or fluorescent nanoparticle and a first ionic surfactant, the nanoparticle functionalised with an affinity probe for binding to said bacterial cells trapped or retained on the surface of the substrate via affinity binding;
- and
- c) a second aqueous solution for washing the unbound nanoparticle from the porous substrate;
- wherein the bacterial cells in the sample are quantifiable by a colorimetric and/or fluorescence output emitted from the nanoparticle bound to the bacterial cells.
Dependencies are inferred from the claim text.