PoC test system and method

A test method for the detection of at least one analyte from a sample fluid in a first sample chamber and a second sample chamber, which are separated by a carrier having at least two surfaces, wherein a front side of the carrier points towards the first sample chamber and a rear side of the carrier points towards the second sample chamber, and

• a) at least one receptor molecule is fixed on the front side of a carrier,
• b) at least one free volume is formed over each of the front side and rear side of the carrier and is delimited in each case by a chamber wall,
• c) the first sample chamber has at least two openings, wherein a sample fluid flows through a first opening along a flow gradient over the front side of the carrier to the second opening distanced from the carrier,
• d) the second sample chamber has at least two openings, wherein the sample fluid flows through a first opening in the second sample chamber from the first sample chamber along a flow gradient over the rear side of the carrier,
• e) the second opening of the first sample chamber and the first opening of the second sample chamber are interconnected by a channel,
• f) the second opening of the second sample chamber enables discharge of the fluid,
• g) the free volumes from b.) are formed at least partially with a fluid column,
• h) wherein the flow gradient along the front and rear side of the carrier is provided by means of pressure application and entrains the fluid column from g.).