Method for eliminating interference of theophylline in immunoassay and immunoassay kit
AssigneeShenzhen Mindray Bio-Medical Electronics Co., Ltd.
InventorsKe Li, Chengxiong Zhan, Yuping Zhang
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Abstract
The disclosure relates to a method for eliminating interference of theophylline in immunoassay and an immunodetection kit. The method for eliminating interference of theophylline in immunoassay includes: performing an immunoassay in the presence of a theophylline interference-resistant agent, the theophylline interference-resistant agent having a final concentration of 0.1 mg/mL-100 mg/mL, and being selected as at least one component from a group consisting of free 6-mercaptopurine, 6-thioguanine, 8-azguanine, hypoxanthine, adenine, purine, 2,6,8-trioxypurine, adenine nucleotide, and adenosine. The method and the kit can effectively reduce interference of theophylline on immunoassay results.
Claims21
An immunoassay method, the method comprising:
- providing a blood sample;
- providing an immunoassay kit comprising a substrate, a labeling substance, and a solid phase coated with an immunoassay reactant;
- performing an immunoassay of the blood sample using the immunoassay kit in the presence of a theophylline interference-resistant agent,
- wherein the theophylline interference-resistant agent reduces interference caused by theophylline in the immunoassay;
- wherein the theophylline interference-resistant agent is 6-mercaptopurine, 6-thioguanine, 8-azaguanine, hypoxanthine, purine, or adenosine;
- wherein the theophylline interference-resistant agent has a final concentration of 10 mg/mL to 100-80 mg/mL;
- and
- detecting the level of pro-gastrin-releasing peptide in the blood sample.
A method for reducing interference of theophylline in an immunoassay for detecting pro-gastrin-releasing peptide of a sample, the method comprising:
- performing an immunoassay reaction in the presence of a theophylline interference-resistant agent,
- wherein the theophylline interference-resistant agent reduces interference caused by theophylline in the immunoassay;
- wherein the theophylline interference-resistant agent has a final concentration of 0.1 mg/mL to 80 mg/mL, and
- wherein the theophylline interference-resistant agent is 6-mercaptopurine, 6-thioguanine, 8-azaguanine, hypoxanthine, purine, or adenosine;
- and
- detecting the level of pro-gastrin-releasing peptide in the blood sample.
Dependencies are inferred from the claim text.