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US 12,607,623 B2Granted
Methods for evaluating angiogenesis
AssigneeWashington University
InventorsPrincess Imoukhuede, Yingye Fang
Life of this patent
Estimating the term…
Abstract
Various methods for isolating cell populations from adipocyte tissue and/or blood and quantifying levels of vascular endothelial growth factor receptors (VEGFR) on the isolated cell populations are provided. Also provided are methods of evaluating or modifying angiogenic therapy in a subject.
Claims10
A method of determining the suitability of an anti-angiogenesis treatment in an obese subject and administering the anti-angiogenesis treatment comprising:
- (a) isolating stromal vascular fraction (SVF) from an adipocyte tissue sample obtained from the subject;
- (b) isolating a subpopulation of cells from the SVF using flow cytometry;
- (c) quantifying levels of vascular endothelial growth factor (VEGF) receptors on cells;
- (d) identifying the subject as a candidate for anti-angiogenesis treatment if the levels of VEGF on the isolated subpopulation of cells exceed a threshold of 24,900 VEGFR1/cell or 25,000 VEGFR3/cell;
- and
- (e) treating the subject identified as a candidate with anti-VEGF drug bevacizumab.
3 more elements
Dependencies are inferred from the claim text.