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US 12,606,936 B2Granted
Compositions and methods for making novel T-cell receptors
AssigneeVCreate, Inc.
InventorsBinbin Chen, Ethan Fast
Life of this patent
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Abstract
The disclosure relates generally to the field of T-cell receptors or T-cell receptor mimics, and methods for obtaining novel T-cell receptors or novel T-cell receptor mimics. The compositions and methods described identify pairs of T-cell receptors and antigens. The compositions and methods allow high throughput analysis so that many antigens can be paired with a large repertoire of T-cell receptors.
Claims20
A method for obtaining a T-cell receptor or a T-cell receptor mimic, comprising the steps of:
- mixing a plurality of macrophage cells comprising a first plurality of nucleic acids encoding a repertoire of chimeric T-cell receptors or T-cell receptor mimics with a plurality of antigen presenting cells comprising an optical reporter and a second plurality of nucleic acids encoding an antigen library, wherein the antigens in the antigen library are fusions of a MHC molecule with a peptide from an antigen;
- expressing the plurality of nucleic acids encoding the repertoire of chimeric T-cell receptors in the plurality of macrophage cells;
- expressing the plurality of nucleic acids encoding the antigen library in the plurality of antigen presenting cells;
- isolating a macrophage cell that has phagocytosed an antigen presenting cell;
- and sequencing the first plurality of nucleic acids and the second plurality of nucleic acids.
2 more elements
Dependencies are inferred from the claim text.