Methods for stratification and early detection of advanced adenoma and/or colorectal cancer using DNA methylation markers

A method of detecting methylation statuses of markers, the method comprising:

• converting unmethylated cytosines of a plurality of DNA fragments in a sample into uracils to generate a plurality of converted DNA fragments, wherein the plurality of DNA fragments were obtained from a biological sample;
• sequencing the plurality of converted DNA fragments to generate a plurality of sequence reads, wherein each sequence read corresponds to a converted DNA fragment;
• and
• detecting a methylation status for each of three or more markers identified in the sequence reads,
• wherein a first of the three or more markers is a methylation locus comprising at least a portion of SEQ ID NO. 1, a second of the three or more markers is a methylation locus comprising at least a portion of SEQ ID NO. 2, and a third of the three or more markers is a methylation locus comprising at least a portion of SEQ ID NO. 3.