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US 12,606,864 B2Granted
Compositions, kits and methods for isolating target polynucleotides
AssigneeDNAe Diagnostics Limited
InventorsNorm Nelson, David Wooldridge
Life of this patent
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Abstract
Provided herein are oligomers, compositions, kits, and methods for capturing target polynucleotides, e.g., for downstream applications such as amplification, library preparation, or sequencing. In some embodiments, a capture oligomer is provided or used that comprises a capture sequence that is annealed to a complement that prevents capture until the complement is displaced in a target-polynucleotide dependent manner. In some embodiments, an amount of target polynucleotide is captured that is less than or equal to a predetermined amount.
Claims9
A capture oligomer comprising, in the 5′ to 3′ direction:
- a capture sequence,
- an internal extension blocker,
- a complement of the capture sequence,
- a fourth additional sequence comprising an adaptor sequence;
- and
- a target-hybridizing sequence,
- wherein the complement of the capture sequence is configured to anneal to the capture sequence in the absence of an extended target sequence annealed to the target-hybridizing sequence and the complement of the capture sequence.
4 more elements
Dependencies are inferred from the claim text.