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US 12,606,860 B2Granted
Systems and methods for processing RNA from cells
Assignee10x Genomics, Inc.
InventorsLuigi Jhon Alvarado Martinez, Eswar Prasad Ramachandran Iyer, Jason Bell
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Abstract
Provided herein are methods, compositions, and systems for processing nucleic acids from individual cells or cell populations. Cells are co-partitioned and processed, allowing for analysis of a variety of types of RNA from cells while minimizing ribosomal RNA species. The present invention enables high-throughput measurement of full length RNA from single cells with a streamlined workflow.
Claims28
A method for assaying ribonucleic acid (RNA) molecules, comprising:
- (a) providing a cell or nucleus that comprises ribonucleic acid (RNA) molecules;
- (b) depleting ribosomal RNA (rRNA) molecules from said RNA molecules in said cell or nucleus, to yield a plurality of remaining RNA molecules;
- (c) fragmenting, in said cell or nucleus, said plurality of remaining RNA molecules to yield a plurality of RNA fragments;
- (d) providing a plurality of nucleic acid barcode molecules, wherein nucleic acid barcode molecules of said plurality of nucleic acid barcode molecules comprise a common barcode sequence;
- and
- (e) using an RNA fragment of said plurality of RNA fragments and a nucleic acid barcode molecule of said plurality of nucleic acid barcode molecules to generate a barcoded nucleic acid molecule.
3 more elements
Dependencies are inferred from the claim text.