Loading patent…
AssigneeProgeneer, Inc.
InventorsSo Yeon Ahn, Srivithya Vellampatti Krishnamoorthy, Soong Ho Um, Sung Oh, Seung Won Shin
Estimating the term…
This application provides methods and compositions related to constructing nucleic acid hydrogels (e.g., RNA hydrogels) having repetitive monomer units, each monomer unit includes one or more G-quadruplex sequences. These G-quadruplex sequences cross-link the nucleic acid concatemer such that it self-assembles into a hydrogel under appropriate conditions. In some embodiments, each monomeric unit of the nucleic acid concatemer comprises a coding sequence for polypeptide of interest; and the nucleic acid hydrogel formed by the nucleic acid concatemer can be used for expressing the polypeptide in high quantities. In some embodiments, at least two RNA concatemers comprising G-quadruplex sequences are produced, one further comprising a spacer and the other further comprising a sequence encoding a polypeptide of interest. These two RNA concatemers are combined and self assembled to form a single, wideband RNA hydrogel.
A first circular DNA template comprising (i) a first promoter sequence and (ii) a sequence complementary to a first G-quadruplex motif, wherein the first G-quadruplex motif comprises one or more sequence as set forth in SEQ ID NOs:
A kit for expressing a polypeptide of interest, wherein the kit comprises
Dependencies are inferred from the claim text.