Method for synthesizing beta-nicotinamide mononucleotide and intermediate thereof
AssigneeFujian Ribio Technology Co., Ltd.
InventorsYonghong Yuan, Qi Pan, Xiaoxue Guo, Xusheng Zhao
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Abstract
The present disclosure relates to a synthesis method of β-nicotinamide mononucleotide (β-NMN) and an intermediate thereof. In the present disclosure, phospholipid metabolism enzymes phospholipase D (PLD) and phospholipase C (PLC) widely present in the biosphere are used as catalysts to prepare β-NMN through two-step enzymolysis or one-pot synthesis; and an intermediate, namely phosphatidyl nicotinamide riboside (PNR), is obtained during the two-step enzymolysis. The present disclosure has simple reaction steps, low production cost, and environmental friendliness, and is suitable for large-scale industrial production.
Claims16
A synthesis method of β-nicotinamide mononucleotide (β-NMN), wherein the synthesis method is conducted by a method selected from the group consisting of (a) and (b):
- (a):
- two-step enzymolysis, comprising:
- a1:
- contacting nicotinamide riboside (NR) and a phospholipid with a phospholipase D (PLD) in the presence of a calcium ion to conduct a catalytic reaction to produce phosphatidyl nicotinamide riboside (PNR);
- and
- a2:
- contacting the PNR produced in step a1 with a phospholipase C (PLC) in the presence of a calcium ion to conduct a catalytic reaction to produce the β-NMN;
- (b):
- one-pot synthesis to prepare the β-NMN,
- wherein the one-pot synthesis to prepare the β-NMN is conducted by a one-pot synthesis method selected from the group consisting of (1) and (2):
- (1):
- combining NR, a phospholipid, and a calcium ion, and adding a PLD to allow a first catalytic reaction to produce PNR, and after the first reaction is completed, adding a PLC to allow a second reaction to obtain the β-NMN;
- (2):
- combining NR, a phospholipid, and a calcium ion, and adding a PLD and a PLC together to allow a catalytic reaction to obtain the β-NMN.
Dependencies are inferred from the claim text.