Production of short chain fatty acids

Extracted microbial lipid comprising triacylglycerol (TAG) molecules, each TAG molecule having a fatty acid esterified at each of an sn-1 position, an sn-2 position and an sn-3 position, wherein at least some of the TAG molecules comprise a short chain fatty acid (SCFA) esterified at the sn-3 position of the TAG molecule, such that at least 1% by weight or by mol %, or both, of the total fatty acid content of the extracted lipid is SCFA, and wherein the esterified SCFAs at the sn-3 position of the TAG molecules comprise a) C4:0, or b) C4:0 and C6:0, wherein the extracted lipid is extracted from a microbial cell comprising one or more exogenous polynucleotide(s) encoding a fatty acid acyltransferase or more than one fatty acid acyltransferase, wherein at least one acyltransferase has activity on SCFA-CoA molecules as a substrate, and wherein each exogenous polynucleotide is operably linked to one or more promoters that are capable of directing expression of said polynucleotide in the cells.