Conversion of lignin-derived monomers to muconate by engineered pseudomonas
AssigneeAlliance for Energy Innovation, LLC
InventorsChristopher W. Johnson, Gregg Tyler Beckham, Allison Jean Zimont Werner
Life of this patent
Estimating the term…
Abstract
Disclosed herein are engineered Pseudomonas useful to relieve the metabolic bottleneck of 4-hydroxybenzoate transformation in a muconate accumulating strain on an engineered Pseudomonas by swapping its endogenous para-hydroxybenzoate-3-hydroxylase (PHBH), PobA, with a homolog, PraI, that has a broader cofactor preference.
Claims4
An engineered Pseudomonas bacterium comprising a gene encoding an exogenous PraI, wherein the bacterium is free of a gene encoding an endogenous PobA and wherein the bacterium increases production of muconic acid from p-coumarate when compared to a non-engineered Pseudomonas bacterium that comprises a gene encoding an endogenous PobA and wherein the bacterium produces at least 40 g/L of muconic acid;
- and wherein the gene encoding for PraI is greater than 85% identical to SEQ ID NO:
- 3;
- and wherein the bacterium has increased production of beta-ketoadipic acid when compared to a non-engineered Pseudomonas bacterium that comprises a gene encoding endogenous PobA.
An engineered Pseudomonas bacterium comprising a gene encoding an exogenous PraI, wherein the bacterium is free of a gene encoding an endogenous PobA and is free of a gene encoding an endogenous PcaIJ and wherein the bacterium increases production of muconic acid from p-coumarate when compared to a non-engineered Pseudomonas bacterium that comprises a gene encoding an endogenous PobA and a gene encoding an endogenous PcaIJ and wherein the bacterium produces at least 40 g/L of muconic acid;
- and wherein the gene encoding for PraI is greater than 85% identical to SEQ ID NO:
- 3;
- and wherein the bacterium has increased production of beta-ketoadipic acid when compared to a non-engineered Pseudomonas bacterium that comprises a gene encoding endogenous PobA and a gene encoding endogenous PcaIJ and is free of a gene encoding exogenous PraI.
Dependencies are inferred from the claim text.