Process for xylitol production

An improved process for high titer xylitol production from pure xylose, the process comprising:

• i. inoculating a thermotolerant and inhibitor tolerant yeast strain to a culture medium;
• ii. incubating the strain in the culture medium of step (i) at a temperature of 28° C. at 200 rpm for a duration of 24 hours and increasing the temperature to 37° C.;
• iii. continuously adding glucose and inducer in the medium in pulse feed of every after 6 hours and maintaining aeration at 1VVM and pH at 5;
• and
• iv. obtaining xylitol from the medium.