Engineered viral capsid polypeptides and uses thereof
AssigneePresident and Fellows of Harvard College
InventorsEric Kelsic, Sam Sinai, George M. Church
Life of this patent
Estimating the term…
Abstract
The technology described herein provides variant adeno-associated viral capsid polypeptides and viruses comprising the same. Further provided herein are methods for delivering a viral payload using viruses comprising variant capsid polypeptides described herein. Described herein are viral vectors comprising a variant sequence of the capsid gene, VP1. In particular, viral vectors with capsid polypeptide mutations that modify tropism of the viral particles relative to particles with wild-type capsid polypeptide are described.
Claims21
A viral capsid polypeptide having a Levenshtein distance to SEQ ID NO:
- 2 of at least 2 and bearing a mutation relative to SEQ ID NO:
- 2 (WT AAV2) in a region corresponding to amino acids 561-588, wherein the mutation comprises an amino acid substitution at amino acid 587.
An AAV VP1 capsid polypeptide having a Levenshtein distance to SEQ ID NO:
- 2 of at least 2 and bearing a mutation in the region corresponding to amino acids 561-588 of SEQ ID NO:
- 2, wherein the mutation comprises an amino acid substitution at amino acid 587.
A viral capsid polypeptide comprising a region corresponding to the amino acid sequence of SEQ ID NO:
- 3 comprising a mutation relative to SEQ ID NO:
- 3, wherein the region comprises an amino acid substitution at position 587 corresponding to SEQ ID NO:
- 2 and is selected from SEQ ID NO:
- 4-211,872.
Dependencies are inferred from the claim text.