Method for producing nucleic acid-encapsulated AAV hollow particle
AssigneesNippon Medical School Foundation, Takara Bio Inc.
InventorsTakashi Okada, Junichi Mineno, Hideto Chono
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Abstract
A method for producing a nucleic acid-encapsulated adeno-associated virus (AAV) hollow particle, comprising the following steps: (1) preparing a linear nucleic acid fragment comprising a sequence for A region and a sequence for D′ region in an AAV inverted terminal repeat (ITR) (i.e., an AD sequence) or a sequence complementary to the AD sequence and a target gene sequence; (2) introducing the nucleic acid fragment prepared in step (1) into a cell capable of producing an AAV hollow particle; and (3) culturing the cell obtained in step (2).
Claims17
A method for producing an empty adeno-associated virus (AAV) particle encapsulating a nucleic acid, the method comprising:
- (1) preparing a linear nucleic acid fragment containing an AD sequence consisting of region A and region D′ in an AAV inverted terminal repeat sequence (ITR) adjacent to each other, or a complementary sequence of the AD sequence, and a sequence of a gene of interest, wherein the AD sequence or the complementary sequence of the AD sequence does not anneal intramolecularly,
- (2) introducing the nucleic acid fragment prepared in step (1) into a cell that produces an empty AAV particle, and
- (3) culturing the cell obtained in step (2).
A linear nucleic acid fragment containing an AD sequence consisting of region A and region D′ in an AAV inverted terminal repeat sequence (ITR) adjacent to each other, or a complementary sequence of the AD sequence, and a sequence of a gene of interest, wherein the AD sequence or the complementary sequence of the AD sequence does not anneal intramolecularly.
Dependencies are inferred from the claim text.