Mutant filamentous fungus, and method for producing protein using same
AssigneeKao Corporation
InventorsToshiharu Arai, Sakurako Ichinose
Life of this patent
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Abstract
A method for producing a mutant filamentous fungus. The method comprises modifications of XYR1 and ACE3 expression in the parent filamentous fungus. The modification of XYR1 is substitution, deletion, insertion, or addition of at least one amino acid residue in a region corresponding to positions 810 to 833 of SEQ ID NO: 1 in a polypeptide that consists of the amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having at least 90, identity thereto and functions as a transcriptional activator of cellulase and hemicellulase, and the modification of ACE3 expression is enhanced expression of a partial polypeptide of ACE3.
Claims20
A method for producing a mutant filamentous fungus, comprising:
- modifying Xylanase Regulator 1 (XYR1) and Activator of Cellulase Expression 3 (ACE3) expression in a parent filamentous fungus, wherein
- the modification of XYR1 is substitution, deletion, insertion, or addition of at least one amino acid residue in a region corresponding to positions 810 to 833 of SEQ ID NO:
- 1 in a polypeptide that consists of SEQ ID NO:
- 1 or an amino acid sequence having at least 90% identity thereto and functions as a transcriptional activator of cellulase and hemicellulase, and
- the modification of ACE3 expression is enhanced expression of a polypeptide that consists of the amino acid sequence at positions 107 to 734 of SEQ ID NO:
- 3 or an amino acid sequence having at least 90% identity thereto, wherein
- if the substitution of at least one amino acid residue in XYR1 is a substitution of Ala at a position corresponding to position 824 of SEQ ID NO:
- 1, the substitution is with Glu, Ile, Leu, Lys, Phe, Thr, Trp, or Tyr, and
- if the substitution of at least one amino acid residue in XYR is a substitution of Val at a position corresponding to position 821 of SEQ ID NO:
- 1, the substitution is with Lys, Trp, or Tyr.
A mutant filamentous fungus comprising modified Xylanase Regulator 1 (XYR1) and showing enhanced expression of a partial polypeptide of Activator of Cellulase Expression 3 (ACE3) compared to a parent filamentous fungus, wherein
- the modified XYR1 is a polypeptide having a substitution, deletion, insertion or addition of at least one amino acid residue in a region corresponding to positions 810 to 833 of SEQ ID NO:
- 1 in the amino acid sequence of SEQ ID NO:
- 1 or an amino acid sequence having at least 90% identity thereto, and the polypeptide functioning as a transcriptional activator of cellulase and hemicellulase, and
- the partial polypeptide of ACE3 is a polypeptide consisting of the amino acid sequence at positions 107 to 734 of SEQ ID NO:
- 3 or an amino acid sequence having at least 90% identity thereto, wherein
- if the substitution of at least one amino acid residue in XYR1 is a substitution of Ala at a position corresponding to position 824 of SEQ ID NO:
- 1, the substitution is with Glu, Ile, Leu, Lys, Phe, Thr, Trp, or Tyr, and
- if the substitution of at least one amino acid residue in XYR is a substitution of Val at a position corresponding to position 821 of SEQ ID NO:
- 1, the substitution is with Lys, Trp, or Tyr.
Dependencies are inferred from the claim text.