Auto-induction regulatory system based on quorum sensing and application thereof
AssigneeOcean Universrry of China
InventorsXiangzhao Mao, Zhen Liu, Zhuoning Cao
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AssigneeOcean Universrry of China
InventorsXiangzhao Mao, Zhen Liu, Zhuoning Cao
Estimating the term…
Disclosed is an auto-induction regulatory system based on quorum sensing, comprising luxI, luxR and egfp, wherein, the promoter for controlling the expression of luxI and luxR is selected from PluxI, PBB or PJ23100; the promoter for controlling the expression of egfp is selected from PluxI, PluxI(T-38C) or PluxI(C-77T). Also disclosed are an application of the auto-induction regulatory system based on quorum sensing in the automatic regulation of expression of a target gene of engineered Escherichia coli, as well as an application thereof in the preparation of alginate lyase and esterase. Further disclosed are a recombinant expression vector and a recombinant engineered bacterium comprising the auto-induction regulatory system based on quorum sensing.
An auto-induction regulatory system based on quorum sensing, wherein:
A recombinant expression vector, whose structure is PluxI-luxI-luxR-PluxI(C-77T)-est7, wherein, the nucleotide sequence of said PluxI is as set forth in SEQ ID NO:
Dependencies are inferred from the claim text.