Self-diversifying systems and methods of use thereof
AssigneesThe Board Institute, Inc., Massachusetts Institute of Technology
InventorsFeng Zhang, Jonathan Leo Schmid-Burgk
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Abstract
A diversity-generating system comprising: a polynucleotide comprising: a variable region within a sequence encoding a protein or polypeptide, wherein the system is configured to generate a library of diversified molecules with an efficiency of 80% as measured by number of the diversified molecules in total number of molecules. The system may further comprise a template region; and a reverse transcriptase or functional domain thereof.
Claims19
A self-diversifying system comprising an in vitro engineered vector, wherein the vector comprises a promoter, a polynucleotide sequence, and a nucleic acid template region, wherein the polynucleotide sequence is operably linked to the promoter, is the nucleotide sequence selected from SEQ ID Nos. 98-123, and comprises one or more open reading frames, wherein at least one of the open reading frames comprises a self-diversifying element, wherein the nucleic acid template region is operably linked to a nucleic acid variable region of the polynucleotide sequence, and wherein the self-diversifying element is a diversity generating retroelement (DGR) or a non-DGR.
Dependencies are inferred from the claim text.