Cleaning performance on protein sensitive soilings VI
AssigneeHenkel AG & Co. KGaA
InventorsChristian Degering, Susanne Wieland, Fabian Falkenberg, Nina Mussmann
Life of this patent
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Abstract
Proteases may include an amino acid sequence having at least 70% sequence identity with the amino acid sequence given in SEQ ID NO:1 over its entire length and, in each case based on the numbering according to SEQ ID NO:1. The protease may have (i) amino acid substitutions, such as 9T, 133A, 144K, 252T and 271E, at the positions corresponding to positions 9, 133, 144, 252 and 271; and (ii) at least one further amino acid substitution at at least one of the positions corresponding to positions 3, 4, 82, 156, 162 or 218. The production and use of said proteases help to improve cleaning performance.
Claims11
A composition, comprising:
- a protease with a conservatively mutated amino acid sequence having at least 95% sequence identity with the amino acid sequence of SEQ ID NO:1 over its entire length, in each case based on the numbering according to SEQ ID NO:1, wherein the protease comprises:
- (i) amino acid substitutions at the positions corresponding to positions 9, 133, 144, 252, and 271;
- wherein, the substitutions at positions 133 and 252 are 133A and 252T;
- and,
- (ii) at least one further amino acid substitution at at least one of the positions corresponding to positions 3, 4, 82, 156, 162, 218, and combinations thereof;
- and,
- wherein the protease exhibits improved cleaning performance when compared to the protease of SEQ ID NO:1.
Dependencies are inferred from the claim text.